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In Vivo Functional Imaging of the Olfactory Bulb at Single-Cell Resolution

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Neuronal Network Analysis

Part of the book series: Neuromethods ((NM,volume 67))

Abstract

Functional properties of neuronal circuits can be best studied in vivo in the living mammalian brain. The use of optical methods, like two-photon calcium imaging, permits analyses of network function at single-cell resolution. This chapter provides a step-by-step description of this technique. Using mouse olfactory bulb as a model system, we compare the performance of genetically encoded calcium sensor TN-XXL and small-molecule calcium indicators; describe how to choose the right calcium indicator and how to load it into the cells of interest; discuss the use of cell type-specific markers and, finally, illustrate the application of this technique for high-resolution in vivo imaging of sensory-driven neuronal activity.

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Acknowledgments

We thank A. Weible, S. Kasperek, G. Heck, and K. Schoentag for technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 596, GA 654/1-1, SFB 870), EU FP7, and the NIH NS DC005259-39.

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Authors and Affiliations

  1. Department of Physiology II, University of Tuebingen, Tuebingen, Germany

    Stefan Fink, Yury Kovalchuk, Bernd Schwendele & Olga Garaschuk

  2. Department of Physiology, Yale University, New Haven, CT, USA

    Ryota Homma & Lawrence B. Cohen

  3. Max-Planck-Institute of Neurobiology, Martinsried, Germany

    Stephan Direnberger & Oliver Griesbeck

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  1. Stefan Fink
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  2. Yury Kovalchuk
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  3. Ryota Homma
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  4. Bernd Schwendele
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  5. Stephan Direnberger
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  6. Lawrence B. Cohen
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  7. Oliver Griesbeck
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  8. Olga Garaschuk
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Editors and Affiliations

  1. , Dpt. Neuroscience & Brain Technologies, Istituto Italiano di Tecnologia (IIT), Via Morego 30, Genova, 16163, Italy

    Tommaso Fellin

  2. , Department of Psychiatry, Massachusetts General Hospital, Fruit St. 55, Boston, 02114, Massachusetts, USA

    Michael Halassa

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Fink, S. et al. (2011). In Vivo Functional Imaging of the Olfactory Bulb at Single-Cell Resolution. In: Fellin, T., Halassa, M. (eds) Neuronal Network Analysis. Neuromethods, vol 67. Humana Press. https://doi.org/10.1007/7657_2011_1

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  • DOI: https://doi.org/10.1007/7657_2011_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-632-6

  • Online ISBN: 978-1-61779-633-3

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