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Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors

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Bioreactors in Stem Cell Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2436))

Abstract

Extracellular vesicles (EVs) are particles with 100–1000 nm sizes which are secreted by cells for intercellular communication. Meanwhile, studies have found that EVs secreted by human stem cells carry similar characteristics (microRNAs, proteins, metabolites, etc.) from their cell counterpart. Thus, EVs derived from stem cells, especially human induced pluripotent stem cells (hiPSCs) and human mesenchymal stromal/stem cells (hMSCs) are promising candidates for cell-free therapy. However, conventional planar culture is insufficient to produce a large amount of cells or EVs to satisfy clinical requirements. In this chapter, we described feasible approaches to harvest EVs secreted by lineage-specific hiPSCs and undifferentiated hMSCs in suspension bioreactors. Differentiation of hiPSCs to cortical organoids can be performed in suspension bioreactors and the corresponding EVs can be isolated and purified. This scale-up protocol can be applied to a majority of stem cell types with EV collection thus provides useful information for both experimental and biomanufacturing purposes.

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Acknowledgments

This work was supported by National Science Foundation (Nos. 1652992 and 1743426) as well as partially by the National Institutes of Health (NIH; R01NS102395). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

Author information

Authors and Affiliations

  1. Department of Chemical & Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA

    Xuegang Yuan, Xingchi Chen & Yan Li

  2. The National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA

    Xuegang Yuan

  3. Department of Industrial & Manufacturing Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, USA

    Changchun Zeng

  4. Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, USA

    David G. Meckes Jr

Authors
  1. Xuegang Yuan
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  2. Xingchi Chen
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  3. Changchun Zeng
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  4. David G. Meckes Jr
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  5. Yan Li
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Corresponding author

Correspondence to Yan Li .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Yuan, X., Chen, X., Zeng, C., Meckes, D.G., Li, Y. (2021). Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 2436. Humana, New York, NY. https://doi.org/10.1007/7651_2021_416

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  • DOI: https://doi.org/10.1007/7651_2021_416

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2017-5

  • Online ISBN: 978-1-0716-2018-2

  • eBook Packages: Springer Protocols

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