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Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells

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Induced Pluripotent Stem Cells and Human Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2549))

Abstract

Differentiating human induced pluripotent stem cells (iPSCs) into multipotent mesenchymal stem/stromal cells (MSCs) offers a renewable source of therapeutically invaluable cells. However, the process of MSC derivation from iPSCs suffers from an undesirably low efficiency. In this chapter, we present an optimized procedure to produce MSCs from human iPSCs with a high efficiency. The protocol depends on the generation of embryoid bodies (EBs) and requires the treatment of EBs with transforming growth factor beta 1 (TGF-β1). The resulting MSCs can be purified based on the expression of CD73, CD105, and CD90 markers and expanded for multiple passages without losing their characteristics.

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Abbreviations

DPBS:

Dulbecco’s phosphate buffered saline

EB:

Embryoid body

FACS:

Fluorescence-activated cell sorting

FBS:

Fetal bovine serum

iPSC:

Induced pluripotent stem cell

MSC:

Mesenchymal stem/stromal cell

TGF-β1:

Transforming growth factor beta 1

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Acknowledgments

We thank the Gates Frontiers Fund and the Ehlers Danlos Center of Excellence at the University of Colorado Anschutz Medical Campus.

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Authors and Affiliations

  1. Department of Dermatology, University of Colorado School of Medicine, Aurora, CO, USA

    Shennea S. McGarvey, Michael Ferreyros, Igor Kogut & Ganna Bilousova

  2. Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Aurora, CO, USA

    Shennea S. McGarvey, Michael Ferreyros, Igor Kogut & Ganna Bilousova

  3. Linda Crnic Institute for Down Syndrome, University of Colorado School of Medicine, Aurora, CO, USA

    Ganna Bilousova

Authors
  1. Shennea S. McGarvey
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  2. Michael Ferreyros
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  3. Igor Kogut
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  4. Ganna Bilousova
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Corresponding author

Correspondence to Ganna Bilousova .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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McGarvey, S.S., Ferreyros, M., Kogut, I., Bilousova, G. (2021). Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_383

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  • DOI: https://doi.org/10.1007/7651_2021_383

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2584-2

  • Online ISBN: 978-1-0716-2585-9

  • eBook Packages: Springer Protocols

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