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Decellularized Scaffolds and Organogenesis pp 11–23Cite as

Decellularized Liver Scaffold for Liver Regeneration

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

After being initially hailed as the ultimate solution to end-stage organ failure, such as end-stage liver disease (ESLD), engineering of vascularized tissues has stalled because of the need for a well-structured circulatory system that can maintain the cells to be seeded inside the construct.

In the field of regenerative medicine, decellularized scaffolds, derived mainly from various non-autologous whole organs, have become an emerging treatment technique to overcome this obstacle. As a result of significant progress made in recent years, organogenesis through whole-organ decellularization scaffolds may now become more feasible than ever before. In this chapter, we describe in detail the necessary steps for liver organogenesis using a decellularized acellular scaffold (DAS), seed cell isolation, and recellularization in a bioreactor-like culture system. This new technique to re-engineer organs may have major implications for the fields of drug discovery, organ transplantation, and ultimately regenerative medicine.

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References

  1. Guyette JP, Charest JM, Mills RW et al (2016) Bioengineering human myocardium on native extracellular matrix. Circ Res 118:56–72

    Article  CAS  Google Scholar 

  2. Uygun BE, Soto-Gutierrez A, Yagi H et al (2010) Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med 16:814–820

    Article  CAS  Google Scholar 

  3. Zhang H, Siegel CT, Li J et al (2016) Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population. J Tissue Eng Regen Med. https://doi.org/10.1002/term.2311

    Article  Google Scholar 

  4. Baptista PM, Moran EC, Vyas D et al (2016) Fluid flow regulation of revascularization and cellular organization in a bioengineered liver platform. Tissue Eng Part C Methods 22:199–207

    Article  CAS  Google Scholar 

  5. Guyette JP, Gilpin SE, Charest JM et al (2014) Perfusion decellularization of whole organs. Nat Protoc 9:1451–1468

    Article  CAS  Google Scholar 

  6. Song JJ, Guyette JP, Gilpin SE et al (2013) Regeneration and experimental orthotopic transplantation of a bioengineered kidney. Nat Med 19:646–651

    Article  CAS  Google Scholar 

  7. Bartlett DC, Hodson J, Bhogal RH et al (2014) Combined use of N-acetylcysteine and liberase improves the viability and metabolic function of human hepatocytes isolated from human liver. Cytotherapy 16:800–809

    Article  CAS  Google Scholar 

  8. Zhang H, Siegel CT, Shuai L et al (2016) Repair of liver mediated by adult mouse liver neuro-glia antigen 2-positive progenitor cell transplantation in a mouse model of cirrhosis. Sci Rep 6:21783–21797

    Article  CAS  Google Scholar 

  9. Caralt M, Velasco E, Lanas A et al (2014) Liver bioengineering: from the stage of liver decellularized matrix to the multiple cellular actors and bioreactor special effects. Organogenesis 10:250–259

    Article  Google Scholar 

  10. Sabetkish S, Kajbafzadeh AM, Sabetkish N et al (2015) Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix liver scaffolds. J Biomed Mater Res A 103:1498–1508

    Article  Google Scholar 

  11. Kobayashi M, Inoue K, Warabi E et al (2005) A simple method of isolating mouse aortic endothelial cells. J Atheroscler Thromb 12:138–142

    Article  Google Scholar 

  12. Watt SM, Gullo F, van der Garde M et al (2013) The angiogenic properties of mesenchymal stem/stromal cells and their therapeutic potential. Br Med Bull 108:25–53

    Article  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC) grant 81570573 to L.H.B. and University Southwestern Hospital grant SHW2014 LC01 to L.H.B.

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Authors and Affiliations

  1. Hepatobiliary Institute, Southwest Hospital, Third Military Medical University, No. 30 Gaotan Yan, ShapingBa District, Chongqing, 400038, China

    Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang & Lianhua Bai

Authors
  1. Wei Yang
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  2. Renpei Xia
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  3. Yujun Zhang
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  4. Hongyu Zhang
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  5. Lianhua Bai
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Corresponding author

Correspondence to Lianhua Bai .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Yang, W., Xia, R., Zhang, Y., Zhang, H., Bai, L. (2017). Decellularized Liver Scaffold for Liver Regeneration. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_53

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  • DOI: https://doi.org/10.1007/7651_2017_53

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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