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Organoids pp 249–254Cite as

Study Bacteria–Host Interactions Using Intestinal Organoids

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

The intestinal epithelial cells function to gain nutrients, retain water and electrolytes, and form an efficient barrier against foreign microbes and antigens. Researchers employed cell culture lines derived from human or animal cancer cells as experimental models in vitro for understanding of intestinal infections. However, most in vitro models used to investigate interactions between bacteria and intestinal epithelial cells fail to recreate the differentiated tissue components and structure observed in the normal intestine. The in vitro analysis of host–bacteria interactions in the intestine has been hampered by a lack of suitable intestinal epithelium culture systems. Here, we present a new experimental model using an organoid culture system to study bacterial infection.

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Acknowledgements

We would like to acknowledge the NIDDK grant R01 DK105118 (J.S.).

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Authors and Affiliations

  1. Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago, 840 S Wood Street, Chicago, IL, 60612, USA

    Yong-guo Zhang

  2. Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago, 840 S Wood Street, Room 704 CSB, Chicago, IL, 60612, USA

    Jun Sun Ph.D., A.G.A.F.

Authors
  1. Yong-guo Zhang
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  2. Jun Sun Ph.D., A.G.A.F.
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Corresponding author

Correspondence to Jun Sun Ph.D., A.G.A.F. .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Zhang, Yg., Sun, J. (2016). Study Bacteria–Host Interactions Using Intestinal Organoids. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_6

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  • DOI: https://doi.org/10.1007/7651_2016_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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