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De Novo Assembly of Plasmids Using Yeast Recombinational Cloning

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The Genetic Manipulation of Staphylococci

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1373))

Abstract

Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Rutgers University, 76 Lipman Dr., New Brunswick, NJ, 08904, USA

    Ameya A. Mashruwala & Jeffrey M. Boyd Ph.D.

Authors
  1. Ameya A. Mashruwala
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  2. Jeffrey M. Boyd Ph.D.
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Corresponding author

Correspondence to Jeffrey M. Boyd Ph.D. .

Editor information

Editors and Affiliations

  1. University of Kansas Medical Center, Kansas City, Kansas, USA

    Jeffrey L. Bose

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© 2015 Springer Science+Business Media New York

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Mashruwala, A.A., Boyd, J.M. (2015). De Novo Assembly of Plasmids Using Yeast Recombinational Cloning. In: Bose, J. (eds) The Genetic Manipulation of Staphylococci. Methods in Molecular Biology, vol 1373. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_275

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  • DOI: https://doi.org/10.1007/7651_2015_275

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3157-6

  • Online ISBN: 978-1-4939-3158-3

  • eBook Packages: Springer Protocols

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