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Particle Sedimentation in pH-Gradients

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Analytical Ultracentrifugation VIII

Part of the book series: Progress in Colloid and Polymer Science ((PROGCOLLOID,volume 131))

Abstract

A new method is explored, where charged colloidal particles are sedimented in a pH-gradient, and an application in following the sedimentation velocity behavior of colloidal gold particles with cationic resp. anionic charge in a pH-gradient is described. A procedure to analyze this type of experiment is developed, where the effects of the changing pH and other parameters resulting from a pH-gradient onto sedimentation are considered. This qualitative analysis allows an understanding of charge effects on the sedimentation behavior of colloids in a preformed pH-gradient and suggests ways for future quantitative evaluation of such data towards the determination of particle charge distributions.

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Acknowledgments

We acknowledge financial support from the Max-Planck Society and the DFG (SFB 448). Dr. Subramanya Mayya is gratefully acknowledged for the colloidal gold dispersions.

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Authors and Affiliations

  1. Department of Colloid Chemistry, Max Planck Institute of Colloids and Interfaces Research Campus Golm, MPI Research Campus Golm, 14424, Potsdam, Germany

    Helmut Cölfen & Gordon Lucas

Authors
  1. Helmut Cölfen
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  2. Gordon Lucas
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Corresponding author

Correspondence to Helmut Cölfen .

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Christine Wandrey Helmut Cölfen

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Cölfen, H., Lucas, G. Particle Sedimentation in pH-Gradients. In: Wandrey, C., Cölfen, H. (eds) Analytical Ultracentrifugation VIII. Progress in Colloid and Polymer Science, vol 131. Springer, Berlin, Heidelberg. https://doi.org/10.1007/2882_013

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