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Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes

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Cell-free Production

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 186))

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Abstract

In vitro biotransformation (ivBT) refers to the use of an artificial biological reaction system that employs purified enzymes for the one-pot conversion of low-cost materials into biocommodities such as ethanol, organic acids, and amino acids. Unshackled from cell growth and metabolism, ivBT exhibits distinct advantages compared with metabolic engineering, including but not limited to high engineering flexibility, ease of operation, fast reaction rate, high product yields, and good scalability. These characteristics position ivBT as a promising next-generation biomanufacturing platform. Nevertheless, challenges persist in the enhancement of bulk enzyme preparation methods, the acquisition of enzymes with superior catalytic properties, and the development of sophisticated approaches for pathway design and system optimization. In alignment with the workflow of ivBT development, this chapter presents a systematic introduction to pathway design, enzyme mining and engineering, system construction, and system optimization. The chapter also proffers perspectives on ivBT development.

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Acknowledgments

We are grateful to Professor Yi-Heng P. Job Zhang, the director of in vitro Synthetic Biology Center of TIB, CAS, for providing scientific suggestions.

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Authors and Affiliations

  1. University of Chinese Academy of Sciences, Beijing, China

    Yanmei Qin, Lin Fan & Xiao Ning

  2. In Vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

    Yanmei Qin, Qiangzi Li, Lin Fan, Xiao Ning, Xinlei Wei & Chun You

  3. University of Chinese Academy of Sciences Sino-Danish College, Beijing, China

    Lin Fan

  4. National Technology Innovation Center of Synthetic Biology, Tianjin, China

    Xinlei Wei & Chun You

Authors
  1. Yanmei Qin
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  2. Qiangzi Li
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  3. Lin Fan
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  4. Xiao Ning
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  5. Xinlei Wei
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  6. Chun You
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Correspondence to Xinlei Wei or Chun You .

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Editors and Affiliations

  1. Department of Chemical Engineering, Tsinghua University, Beijing, China

    Yuan Lu

  2. Department of Bioengineering, Stanford University, Stanford, CA, USA

    Michael C. Jewett

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Qin, Y., Li, Q., Fan, L., Ning, X., Wei, X., You, C. (2023). Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes. In: Lu, Y., Jewett, M.C. (eds) Cell-free Production. Advances in Biochemical Engineering/Biotechnology, vol 186. Springer, Cham. https://doi.org/10.1007/10_2023_231

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