Abstract
Protein N-glycosylation is regulated by the availability of nucleotide sugar substrates. Pels Rijcken et al.[3] have proposed that elevated cytosolic levels of UDP-N-acetylhexosamine impaired the transport of CMPNeuAc into the Golgi, which lead to decreased sialylation. Addition of glucosamine to cell culture medium has resulted in an increase in antennarity of N-glycans [4] . By manipulating the levels of nucleotide sugar precursors available to NS0 cells (by additions to the cell culture medium) a strategy for controlling product glycosylation could be devised.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References and Acknowledgements
Heal, R., McGivan, J. (1998) Induction of calreticulin expression in response to amino acid deprivation in Chinese Hamster Ovary cells. Biochem.J.329 389–394.
Guile, G.R, Rudd, P.M., Wing, D.R., Prime, S.B., Dwek, R.A. (1996) A Rapid high-resolution high performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Anal. Biochem, 240 (2) 210–26.
Pels Rijcken, W.R., Ferwerda, W., Van den Eijden, D.H., Overdijk, B. (1995) Influence ofD-galactosamine on the synthesis of sugar nucleotides and glycoconjugates in rat hepatocytes. Glycobiology 5, 495–502.
Grammatikos, S.I., Valley, U., Nimtz, M., Conradt, H.S., Wagner, R. (1998) Intracellular UDP-N-Acetyl hexosamine Pool Affects N-Glycan Complexity: A Mechanism of Ammonium Action on Protein Glycosylation. Biotechnology Progress 14,410–419.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1999 Kluwer Academic Publishers
About this chapter
Cite this chapter
Hills, A., Patel, A., Boyd, P., James, D. (1999). Control of Therapeutic Monoclonal Antibody Glycosylation. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_58
Download citation
DOI: https://doi.org/10.1007/0-306-46875-1_58
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
eBook Packages: Springer Book Archive