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Morphological and molecular comparison of Myxobolus procerus (Kudo, 1934) and M. intramusculi n. sp. (Myxozoa) parasitising muscles of the trout-perch Percopsis omiscomaycus

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Abstract

Inter- and intracellular cysts from a presumed single species, Myxobolus procerus (Kudo, 1934), parasitising the trout-perch Percopsis omiscomaycus were compared with respect to their spore morphology and small subunit 18S ribosomal DNA. The two cyst types have similar pyriform spores and comparable ranges in spore length and width, size of the polar capsules and number of filament coils. However, fixed spores of the intracellular cysts are significantly shorter (p < 0.05) in mean length than those from intercellular cysts, giving them a broader appearance. Comparing 991 base pairs of 18S rDNA revealed a 20 bp difference between the two cyst types (97.9% similarity), 10 due to base substitutions and 10 due to the presence or absence of a base. The described morphological differences and consistent 2.1% difference in the 18S rDNA reveal that the intracellular form is a separate species, which is described herein as Myxobolus intramusculi n. sp. M. intramusculi n. sp. and M. procerus may be sister species and it is suggested that the two species arose through sympatric speciation involving a switch in site specificity within a single host species.

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References

  • K.B. Andree S.J. Gresoviac R.P. Hedrick (1997) ArticleTitleSmall subunit ribosomal RNA sequences unite alternate actinosporean and myxosporean stages of Myxobolus cerebralis the causative agent of whirling disease in salmonid fish Journal of Eukaryotic Microbiology 44 208–215 Occurrence Handle1:CAS:528:DyaK2sXktV2ktr0%3D Occurrence Handle9183708

    CAS  PubMed  Google Scholar 

  • S.F. Altschul T.L. Madden A.A. Schaffer J. Zhang Z Zhang W. Miller D.J. Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acid Research 25 3389–3402 Occurrence Handle10.1093/nar/25.17.3389 Occurrence Handle1:CAS:528:DyaK2sXlvFyhu7w%3D

    Article  CAS  Google Scholar 

  • S. Bahri K.B. Andree R.P. Hedrick (2003) ArticleTitleMorphological and phylogenetic studies of marine Myxobolus spp. from Mullet in Ichkeul Lake, Tunisia Journal of Eukaryotic Microbiology 50 463–470 Occurrence Handle14733438

    PubMed  Google Scholar 

  • J.L. Bartholomew M.J. Whipple D.G. Stevens J.L. Fryer (1997) ArticleTitleThe life cycle of Ceratomyxa shasta, a myxosporean parasite of salmonids, requires a freshwater polychaete as an alternate host Journal of Parasitology 83 859–868 Occurrence Handle1:CAS:528:DyaK2sXntlOms7w%3D Occurrence Handle9379291

    CAS  PubMed  Google Scholar 

  • D. Cone T. Eurell R. Axler D. Rau V. Beasley (1997) ArticleTitleIntense infections with a variant of Myxobolus procerus (Myxosporea) in muscle of trout-perch (Percopsis omiscomaycus) in Duluth Harbor, Lake Superior Folia Parasitologica 44 7–11 Occurrence Handle1:STN:280:ByiA28nkvFU%3D Occurrence Handle9229571

    CAS  PubMed  Google Scholar 

  • A.O. Dechtiar J.A. Maclean S.J. Nepszy (1989) ArticleTitleParasites of fishes of Algonquin Park lakes Ontario Fisheries Technical Report Series 20 19

    Google Scholar 

  • E. Eszterbauer (2002) ArticleTitleMolecular biology can differentiate morphologically indistinguishable Myxosporean species: Myxobolus elegans and M. hungaricus Acta Veterinaria Hungarica 50 59–62 Occurrence Handle10.1556/AVet.50.2002.1.8 Occurrence Handle12061237

    Article  PubMed  Google Scholar 

  • E. Eszterbauer (2004) ArticleTitleGenetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity Diseases of Aquatic Organisms 58 35–40 Occurrence Handle15038449

    PubMed  Google Scholar 

  • H.G. Guilford (1965) ArticleTitleNew species of Myxosporidia found in Percid fishes from Green Bay (Lake Michigan) Transactions of the American Microscopical Society 84 566–573 Occurrence Handle1:STN:280:CCmD2crpsFc%3D Occurrence Handle4954402

    CAS  PubMed  Google Scholar 

  • S.L. Hallett S.D. Atkinson C. Erséus M. El-Matbouli (2004) ArticleTitleMolecular methods clarify morphometric variation in triactinomyxon spores (Myxozoa) released from different oligochaete hosts Systematic Parasitology 57 1–14 Occurrence Handle10.1023/B:SYPA.0000010682.90311.91 Occurrence Handle14739670

    Article  PubMed  Google Scholar 

  • D.M.L. Hervio M.L. Kent J. Khattra J. Sakanari H. Yokohama R.H Devlin (1997) ArticleTitleTaxonomy of Kudoa species (Myxosporea) using small subunit ribosomal DNA sequence Canadian Journal of Zoology 75 2112–2119 Occurrence Handle1:CAS:528:DyaK1cXhtlSmu70%3D

    CAS  Google Scholar 

  • D.M. Hillis M.T. Dixon (1991) ArticleTitleRibosomal DNA: molecular evolution and phylogenetic inference The Quarterly Review of Biology 66 411–453 Occurrence Handle10.1086/417338 Occurrence Handle1:STN:280:By2C2M%2FmsFc%3D Occurrence Handle1784710

    Article  CAS  PubMed  Google Scholar 

  • R.R. Kudo (1934) ArticleTitleStudies on some protozoan parasites of fishes of Illinois Illinois Biological Monographs 23 1–41

    Google Scholar 

  • D. Lin L.A. Hanson L.M. Pote (1999) ArticleTitleSmall subunit ribosomal RNA sequence of Henneguya exilis (class Myxosporea) identifies the actinosporean stage from an oligochaete host Journal of Eukaryotic Microbiology 46 66–68 Occurrence Handle1:CAS:528:DyaK1MXitFCiuro%3D Occurrence Handle10188262

    CAS  PubMed  Google Scholar 

  • K. Molnár E. Eszterbauer C. Székely Á Dan B. Harrach (2002) ArticleTitleMorphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish Journal of Fish Diseases 24 643–652 Occurrence Handle10.1046/j.1365-2761.2002.00409.x

    Article  Google Scholar 

  • C.V. Nielsen M. Køie C.S. Székely K. Buchmann (2002) ArticleTitleComparative analysis of 18S RNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP Bulletin of the European Association of Fish Pathologists 22 201–205

    Google Scholar 

  • D.L. Swofford (2001) PAUP* Phylogenetic analysis using parsimony (*and other methods), Version 4 Sinauer Associates Sunderland Massachusetts, USA

    Google Scholar 

  • Systat (Version 9). Clecom Software Ltd, The Research Park, Vincent Drive, Edgbaston, Birmingham, B15 2SQ, UK

  • J.D. Thompson T.J. Gibson F. Plewniak F. Jeanmougin D.G. Higgins (1997) ArticleTitleThe Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Research 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876

    Article  Google Scholar 

  • C.M. Whipps R.D. Adlard M.S. Bryant M.L Kent (2003) ArticleTitleTwo unusual myxozoans, Kudoa quadricornis n. sp. (Multivalvulida) from the muscle of godspotted trevally (Carangoides fulvoguttatus) and Kudoa permulticapsula n. sp. (Multivalvulida) from the muscle of spanish mackerel (Scomberomorus commersoni) from the Great Barrier Reef, Australia Journal of Parasitology 89 168–173 Occurrence Handle1:STN:280:DC%2BD3s7ktlOltA%3D%3D Occurrence Handle12659322

    CAS  PubMed  Google Scholar 

  • C. Xiao S.S. Desser (2000) ArticleTitleMolecular characterization of myxozoan parasites from Lake Sasajewun, Algonquin Park, Ontario, by riboprinting Journal of Eukaryotic Microbiology 47 85–89 Occurrence Handle1:CAS:528:DC%2BD3cXpvFOnsA%3D%3D Occurrence Handle10651301

    CAS  PubMed  Google Scholar 

  • H. Yokoyama D. Inoue A. Kumamaru H. Wakabayashi (1997) ArticleTitleMyxobolus koi (Myxozoa:Myxosporea) forms large and small type cysts in the gills of common carp Fish Pathology 32 11–217

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Saint Mary’s University, Halifax, Nova Scotia, B3H 3C3, Canada

    R.H. Easy & D.K. Cone

  2. Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia, B3H 3Z1, Canada

    R.H. Easy & S.C. Johnson

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  1. R.H. Easy
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  2. S.C. Johnson
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  3. D.K. Cone
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Correspondence to R.H. Easy.

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Easy, R., Johnson, S. & Cone, D. Morphological and molecular comparison of Myxobolus procerus (Kudo, 1934) and M. intramusculi n. sp. (Myxozoa) parasitising muscles of the trout-perch Percopsis omiscomaycus. Syst Parasitol 61, 115–122 (2005). https://doi.org/10.1007/s11230-005-3135-9

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