Abstract
Adenylyl cyclase 2 (ADCY2), a class B member of adenylyl cyclases, is important in accelerating phosphor-acidification as well as glycogen synthesis and breakdown. Given its distinct role in flesh tenderization after butchering, we cloned and sequenced the ADCY2 gene from Yanbian cattle and assessed its expression in bovine tissues. A 2947 bp nucleotide sequence representing the full-length cDNA of bovine ADCY2 gene was obtained by 5′ and 3′ remote analysis computations for gene expression. Analyses of the putative protein sequence showed that ADCY2 had high homology among species, except with the non-mammal Oreochromis niloticus. Gene structural domain analyses in humans and rats indicated that the ADCY2 protein had no flaw; only the transmembrane domain was reduced and the CYCc structure domain was shortened. Assessment of ADCY2 expression in bovine tissues by real-time PCR showed that the highest expression was in the testes, followed by the longissimus dorsi, tensor fasciae latae, and latissimus dorsi. These data will serve as a foundation for further insight into the cattle ADCY2 gene.
Similar content being viewed by others
References
Drozdov I, Svejda B, Gustafsson BI, Mane S, Pfragner R, Kidd M, Modlin IM (2011) Gene network inference and biochemical assessment delineates GPCR pathways and CREB targets in small intestinal neuroendocrine neoplasia. PLoS One 6(8):e22457
Iyengar R (1993) Molecular and functional diversity of mammalian Gs-stimulated adenylyl cyclases. FASEB J 7(9):768–775
Hellevuo K, Yoshimura M, Kao M, Hoffman P, Cooper D, Tabakoff B (1993) A novel adenylyl cyclase sequence cloned from the human erythroleukemia cell line. Biochem Biophys Res Commun 192(1):311–318
Hellevuo K, Berry R, Sikela JM, Tabakoff B (1995) Localization of the gene for a novel human adenylyl cyclase (ADCY7) to chromosome 16. Hum Genet 95(2):197–200
Yu SJ, Yu JK, Ge WT, Hu HG, Yuan Y, Zheng S (2011) SPARCL1, Shp2, MSH2, E-cadherin, p53, ADCY-2 and MAPK are prognosis-related in Colorectal cancer. World J Gastroenterol 17(15):2028
Hardin M, Zielinski J, Wan E, Hersh C, Castaldi P, Schwinder E, Hawrylkiewicz I, Sliwinski P, Cho M, Silverman E (2012) CHRNA3/5, IREB2, and ADCY2 are associated with severe COPD in Poland. Am J Respir Cell Mol Biol 47(2):203–208
Edelhoff S, Villacres E, Storm D, Disteche C (1995) Mapping of adenylyl cyclase genes type I, II, III, IV, V, and VI in mouse. Mamm Genome 6(2):111–113
Liu Z, Yan SF, Walker JR, Zwingman TA, Jiang T, Li J, Zhou Y (2007) Study of gene function based on spatial co-expression in a high-resolution mouse brain atlas. BMC Syst Biol 1(1):19
Alves-Costa FA, Figueiredo MA, Lanes CFC, Almeida DV, Marins LF, Wasko AP (2012) A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach. Anais da Academia Brasileira de Ciências 84(2):487–494
Chowdhary B, Frönicke L, Gustavsson I, Scherthan H (1996) Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7(4):297–302
Band MR, Larson JH, Rebeiz M, Green CA, Heyen DW, Donovan J, Windish R, Steining C, Mahyuddin P, Womack JE (2000) An ordered comparative map of the cattle and human genomes. Genome Res 10(9):1359–1368
Amarante M, Gallagher D, Burzlaff J, Lopes C, Womack J, Taylor J, Davis S (1999) Physical mapping of ADCY2, FSHB and HBB to bovine chromosome 15 by FISH of bovine bacterial artificial chromosome clones. Cytogenet Genome Res 85(3–4):271–272
Keele J, Shackelford S, Kappes S, Koohmaraie M, Stone R (1999) A region on bovine chromosome 15 influences beef longissimus tenderness in steers. J Anim Sci 77(6):1364–1371
Rexroad Iii CE, Bennett GL, Stone RT, Keele JW, Fahrenkrug SC, Freking BA, Kappes SM, Smith TPL (2001) Comparative mapping of BTA15 and HSA11 including a region containing a QTL for meat tenderness. Mamm Genome 12(7):561–565
Yuan W, Bernal AL (2007) Cyclic AMP signalling pathways in the regulation of uterine relaxation. BMC Pregnancy Childbirth 7(1):S10
Xin J, Li-chun Z, Zhao-zhi L, Xiao-hui L, Hai-guo J, Chang-guo Y (2011) Association of polymorphisms in the calpain I gene with meat quality traits in Yanbian yellow cattle of China. Asian Aust J Anim Sci 24(1):9–16
Li Y, Jin H, Yan C, Seo K, Zhang L, Ren C, Jin X (2012) Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Mol Biol Rep 39(12):1–7
Gaudin C, Homey CJ, Ishikawa Y (1994) Mammalian adenylyl cyclase family members are randomly located on different chromosomes. Hum Genet 94(5):527–529
Khalyfa A, Buazza M, Qiang H, Xu M, Taylor C, Marshall FJ, Klueber M, Cooper NGF (2007) Gene expression profiling for adult human olfactory neuroepithelial-derived progenitors. Gene Ther Mol Biol 11:203–218
Pronko SP, Saba LM, Hoffman PL, Tabakoff B (2010) Type 7 adenylyl cyclase-mediated hypothalamic-pituitary-adrenal axis responsiveness: influence of ethanol and sex. J Pharmacol Exp Ther 334(1):44–52
Acknowledgments
This work was supported by the Natural Science Foundation of China (Grant No. 31160442), International cooperation projects (NSFC-NRF): Function analysis of the genes with meat quality in Hanwoo and Yanbian cattle (Grant No. 31211140240) and a National Research Foundation of Korea (NRF) Grant (NRF-2012K1A2B1A03000412).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Li, Y.X., Jin, H.G., Yan, C.G. et al. Molecular cloning, sequence identification, and gene expression analysis of bovine ADCY2 gene. Mol Biol Rep 41, 3561–3568 (2014). https://doi.org/10.1007/s11033-014-3167-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-014-3167-9