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Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review

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Abstract

Low-level laser therapy (LLLT) has been used in several in vitro experiments in order to stimulate cell proliferation. Cells such as fibroblasts, keratinocytes, lymphocytes, and osteoblasts have shown increased proliferation when submitted to laser irradiation, although little is known about the effects of LLLT on stem cells. This study aims to assess, through a systematic literature review, the effects of LLLT on the in vitro proliferation of mesenchymal stem cells. Using six different terms, we conducted an electronic search in PubMed/Medline database for articles published in the last twelve years. From 463 references obtained, only 19 papers met the search criteria and were included in this review. The analysis of the papers showed a concentration of experiments using LLLT on stem cells derived from bone marrow, dental pulp, periodontal ligament, and adipose tissue. Several protocols were used to irradiate the cells, with variations on wavelength, power density, radiation time, and state of light polarization. Most studies demonstrated an increase in the proliferation rate of the irradiated cells. It can be concluded that the laser therapy positively influences the in vitro proliferation of stem cells studied, being necessary to carry out further experiments on other cell types and to uniform the methodological designs.

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References

  1. Wu Y, Wang J, Gong D, Gu H, Hu S, Zhang H (2012) Effects of low-level laser irradiation on mesenchymal stem cell proliferation: a microarray analysis. Laser Med Sci 27(2):509–519. doi:10.1007/s10103-011-0995-x

    Article  Google Scholar 

  2. Li WT, Chen HL, Wang CT (2006) Effect of light emitting diode irradiation on proliferation of human bone marrow mesenchymal stem cells. J Med Biol Eng 26(1):35–42

    Google Scholar 

  3. Kreisler M, Christoffers AB, Al-Haj H, Willershausen B, d’Hoedt B (2002) Low-level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts. Lasers Surg Med 30(5):365–369

    Article  PubMed  Google Scholar 

  4. Tuby H, Maltz L, Oron U (2007) Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39(4):373–378

    Article  PubMed  Google Scholar 

  5. Kamali F, Bayat M, Torkaman G, Ebrahimi E, Salavati M (2007) The therapeutic effect of low-level laser on repair of osteochondral defects in rabbit knee. J Photochem Photobiol B 88(1):11–15

    Article  CAS  PubMed  Google Scholar 

  6. Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:4. doi:10.1186/1423-0127-16-4

    Article  PubMed Central  PubMed  Google Scholar 

  7. Eduardo FP, Bueno DF, de Freitas PM, Marques MM, Passos-Bueno MR, Eduardo CP, Zatz M (2008) Stem cell proliferation under low-intensity laser irradiation: a preliminary study. Lasers Surg Med 40(6):433–438. doi:10.1002/lsm.20646

    Article  Google Scholar 

  8. Mvula B, Moore TJ, Abrahamse H (2010) Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells. Lasers Med Sci 25(1):33–39. doi:10.1007/s10103-008-0636-1

    Article  CAS  PubMed  Google Scholar 

  9. Hou JF, Zhang H, Yuan X, Li J, Wei YJ, Hu SS (2008) In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med 40(10):726–733. doi:10.1002/lsm.20709

    Article  PubMed  Google Scholar 

  10. Soleimani M, Abbasnia E, Fathi M, Sahraei H, Fathi Y, Kaka G (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts: an in vitro study. Laser Med Sci 27(2):423–430. doi:10.1007/s10103-011-0930-1

    Article  Google Scholar 

  11. Wilden L, Karthein R (1998) Import of radiation phenomena of electrons and therapeutic low-level laser in regard to mitochondrial energy transfer. J Clin Laser Med Surg 16(3):159–165

    CAS  PubMed  Google Scholar 

  12. Li WT, Leu YC, Wu JL (2010) Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg 28:S157–S165. doi:10.1089/pho.2009.2540

    Article  CAS  PubMed  Google Scholar 

  13. Peng F, Wu H, Zheng Y, Xu X, Yu J (2012) The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Lasers Med Sci 27(3):645–653. doi:10.1007/s10103-011-1005-z

    Article  PubMed  Google Scholar 

  14. Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC (2003) Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci 18(2):95–99. doi:10.1007/s10103-003-0262-x

    Article  PubMed  Google Scholar 

  15. Kim WS, Calderhead RG (2011) Is light-emitting diode phototherapy (LED-LLLT) really effective? Laser Ther 20(3):205–215

    Article  PubMed Central  PubMed  Google Scholar 

  16. Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S, Bar I, Vago R (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20(3–4):138–146

    Article  PubMed  Google Scholar 

  17. Tuby H, Maltz L, Oron U (2009) Implantation of low-level laser irradiated mesenchymal stem cells into the infarcted rat heart is associated with reduction in infarct size and enhanced angiogenesis. Photomed Laser Surg 27(2):227–233. doi:10.1089/pho.2008.2272

    Article  CAS  PubMed  Google Scholar 

  18. Holder MJ, Milward MR, Palin WM, Hadis MA, Cooper PR (2012) Effects of red light-emitting diode irradiation on dental pulp cells. J Dent Res 91(10):961–966

    Article  CAS  PubMed  Google Scholar 

  19. Leonida A, Paiusco A, Rossi G, Carini F, Baldoni M, Caccianiga G (2013) Effects of low-level laser irradiation on proliferation and osteoblastic diferentiation of human mesenchymal stem cells seeded on three-dimensional biomatrix: in vitro pilot study. Lasers Med Sci 28(1):125–132. doi:10.1007/s10103-012-1067-6

    Article  CAS  PubMed  Google Scholar 

  20. Lipovsky A, Oron U, Gedanken A, Lubart R (2013) Low-level visible light (LLVL) irradiation promotes proliferation of mesenchymal stem cells. Lasers Med Sci 28(4):1113–1117. doi:10.1007/s10103-012-1207-z

    Article  PubMed  Google Scholar 

  21. De Villiers JA, Houreld NN, Abrahamse H (2011) Influence of low intensity laser irradiation on isolated human adipose derived stem cells over 72 hours and their differentiation potential into smooth muscle cells using retinoic acid. Stem Cell Rev 7(4):869–882. doi:10.1007/s12015-011-9244-8

    Article  CAS  PubMed  Google Scholar 

  22. Horvát-Karajz K, Balogh Z, Kovács V, HámoriDrrernat A, Sréter L, Uher F (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41(6):463–469. doi:10.1002/lsm.20791

    Article  PubMed  Google Scholar 

  23. Mvula B, Mathope T, Moore T, Abrahamse H (2008) The effect of low-level laser irradiation on adult human adipose-derived stem cells. Lasers Med Sci 23(3):277–282

    Article  CAS  PubMed  Google Scholar 

  24. Ong WK, Chen HF, Tsai CT, Fu YJ, Wong YS, Yen DJ, Chang TH, Huang HD, Lee OK, Chien S, Ho JH (2013) The activation of directional stem cell motility by green light-emitting diode irradiation. Biomaterials 34(8):1911–1920. doi:10.1016/j.biomaterials.2012.11.065

    Article  CAS  PubMed  Google Scholar 

  25. Soares DM, Ginani F, Henriques AH, Barboza CAG (2013) Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci. doi:10.1007/s10103-013-1436-9

    Google Scholar 

  26. Giannelli M, Chellini F, Sassoli C, Francini F, Pini A, Squecco R, Nosi D, Bani D, Zecchi-Orlandini S, Formigli L (2013) Mesenchymal stromal cells with diode laser: Effects and mechanisms of action. J Cell Physiol 228(1):172–181. doi:10.1002/jcp.24119

    Article  CAS  PubMed  Google Scholar 

  27. Pereira LO, Longo JPF, Azevedo RB (2012) Laser irradiation did not increase the proliferation or the differentiation of stem cells from normal and inflamed dental pulp. Arch Oral Biol 57(8):1079–1085. doi:10.1016/j.archoralbio.2012.02.012

    Article  PubMed  Google Scholar 

  28. Wang J, Huang W, Wu Y, Hou J, Nie Y, Gu H, Li J, Hu S, Zhang H (2012) MicroRNA-193 pro-proliferation effects for bone mesenchymal stem cells after low-level irradiation treatment through inhibitor of growth family, member 5. Stem Cells Dev 21(13):2508–2519. doi:10.1089/scd.2011.0695

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Kim HK, Kim JH, Abbas AA, Kim DO, Park SJ, Chung JY, Song EK, Yoon TR (2009) Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24(2):214–222. doi:10.1007/s10103-008-0550-6

    Article  PubMed  Google Scholar 

  30. Bouvet-Gerbettaz S, Merigo E, Rocca JP, Carle GF, Rochet N (2009) Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med 41(4):291–297. doi:10.1002/lsm.20759

    Article  PubMed  Google Scholar 

  31. Sulewski JG (2000) Historical survey of laser dentistry. Dent Clin North Am 44(4):717–752

    CAS  PubMed  Google Scholar 

  32. Evans DH, Abrahamse H (2008) Efficacy of three laser wavelengths for in vitro wound healing. Photodermatol Photoimmunol Photomed 24(4):199–210. doi:10.1111/j.1600-0781.2008.00362.x

    Article  PubMed  Google Scholar 

  33. Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation stimulated cell proliferation in vitro. Lasers Surg Med 36(1):8–12

    Article  PubMed  Google Scholar 

  34. Schindl A, Merwald H, Schindl L, Kaun C, Wojta J (2003) Direct stimulatory effect of low-intensity 670-nm laser irradiation on human endothelial cell proliferation. Br J Dermatol 148(2):334–336

    Article  CAS  PubMed  Google Scholar 

  35. Werneck CE, Pinheiro ALB, Pacheco MTT, Soares CP, de Castro JLF (2005) Laser light is capable of inducing proliferation of carcinoma cells in culture: A spectroscopic in vitro study. Photomed Laser Surg 23(3):300–303

    Article  PubMed  Google Scholar 

  36. De Castro JLF, Pinheiro ALB, Werneck CE, Soares CP (2005) The effect of laser therapy on the proliferation of oral KB carcinoma cells: an in vitro study. Photomed Laser Surg 23(6):586–589

    Article  PubMed  Google Scholar 

  37. Fujihara NA, Hiraki KRN, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38(4):332–336

    Article  PubMed  Google Scholar 

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Ethical statement

The manuscript does not contain clinical studies or patient data.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Fernanda Ginani & Carlos Augusto Galvão Barboza

  2. Postgraduate Program in Dentistry, Federal University of Pernambuco, Recife, PE, Brazil

    Diego Moura Soares

  3. Postgraduate Program in Structural and Functional Biology, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Mardem Portela e Vasconcelos Barreto

  4. Department of Morphology, Center of Biosciences, Federal University of Rio Grande do Norte, Av. Senador Salgado Filho, 3000, Lagoa Nova, Natal, RN, 59072-970, Brazil

    Carlos Augusto Galvão Barboza

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  1. Fernanda Ginani
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  2. Diego Moura Soares
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  3. Mardem Portela e Vasconcelos Barreto
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  4. Carlos Augusto Galvão Barboza
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Correspondence to Carlos Augusto Galvão Barboza.

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Ginani, F., Soares, D.M., Barreto, M.P.V. et al. Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review. Lasers Med Sci 30, 2189–2194 (2015). https://doi.org/10.1007/s10103-015-1730-9

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  • DOI: https://doi.org/10.1007/s10103-015-1730-9

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