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Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus

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Abstract

Papaya lethal yellowing virus (PLYV) causes an economically important disease in papayas in northeastern Brazil. Based on biological and molecular properties, PLYV has been tentatively assigned to the genus Sobemovirus. We report the sequence of the full-length genome of a PLYV isolate from Brazil, determined by deep sequencing. The PLYV genome is 4,145 nt long and contains four ORFs, with an arrangement identical to that of sobemoviruses. The polyprotein and CP display significant sequence identity with the corresponding proteins of other sobemoviruses. Pairwise comparisons and phylogenetic analysis based on complete nucleotide sequences confirm the classification of PLYV in the genus Sobemovirus.

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Author notes

  1. Álvaro J. Pereira

    Present address: Universidade Estadual do Ceará, Itapipoca, CE, 62500-000, Brazil

Authors and Affiliations

  1. Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Álvaro J. Pereira, Renan S. Cascardo & F. Murilo Zerbini

  2. Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Poliane Alfenas-Zerbini

  3. Embrapa Mandioca e Fruticultura, Cruz das Almas, BA, 44380-000, Brazil

    Eduardo C. Andrade

Authors
  1. Álvaro J. Pereira
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  2. Poliane Alfenas-Zerbini
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  3. Renan S. Cascardo
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  4. Eduardo C. Andrade
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  5. F. Murilo Zerbini
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Corresponding author

Correspondence to F. Murilo Zerbini.

Additional information

The sequence reported in this paper has been deposited in the GenBank database under the accession number JX123318.

Á. J. Pereira and P. Alfenas-Zerbini contributed equally to this work.

Electronic supplementary material

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705_2012_1384_MOESM1_ESM.tif

Supplementary Figure S1. Sequence coverage along the genome of papaya lethal yellowing virus. The numbers above the diagram indicate nucleotide positions in the viral genome. Coverage based on a contig assembled from 30,665 reads using Geneious 5.4.5. (TIFF 16 kb)

705_2012_1384_MOESM2_ESM.tif

Supplementary Figure S2. Pairwise nucleotide (A) and deduced amino acid (B-E) sequence identities between papaya lethal yellowing virus (PLYV) and viruses in the genus Sobemovirus. (B) p1 protein, (C) p2a protein, (D) p2b protein, (E) coat protein. (TIFF 4238 kb)

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Pereira, Á.J., Alfenas-Zerbini, P., Cascardo, R.S. et al. Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus . Arch Virol 157, 2009–2011 (2012). https://doi.org/10.1007/s00705-012-1384-x

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  • DOI: https://doi.org/10.1007/s00705-012-1384-x

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