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Narcissus symptomless virus: a new carlavirus of daffodils

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Summary.

A filamentous virus, with particles 600–650 nm long, was purified from Narcissus pseudonarcissus (daffodil) in Hangzhou and an antiserum prepared. After mechanical inoculation, the virus could be detected serologically in Narcissus species but not in some commonly used virus indicators. Infection was symptomless. The complete sequence of the genomic RNA (8281 nt) showed six predicted ORFs typical of carlaviruses. Pairwise comparisons of gene sequences and phylogenetic analysis demonstrated that the new virus should be classified as a carlavirus but that it was not closely related to members of any current species. We propose the name Narcissus symptomless virus (NSV).

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References

  • Adams MJ, Accotto GP, Agranovsky AA, et al. (2005) Flexiviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp 1089–1124

  • MJ Adams JF Antoniw M Bar-Joseph AA Brunt T Candresse GD Foster GP Martelli RG Milne SF Zavriev CM Fauquet (2004) ArticleTitleThe new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation Arch Virol 149 1045–1060 Occurrence Handle15098118 Occurrence Handle1:CAS:528:DC%2BD2cXjt1Sgtr4%3D

    PubMed  CAS  Google Scholar 

  • Anon. (2001) Wisconsin Package version 10.3. Accelrys Inc., San Diego, CA, USA

  • AA Brunt (1995) Narcissus G Loebenstein RH Lawson AA Brunt (Eds) Virus and virus-like diseases of bulb and flower crops J Wiley Chichester, UK 322–334

    Google Scholar 

  • J Chen JP Chen MJ Adams (2002) ArticleTitleCharacterisation of some carla- and potyviruses from bulb crops in China Arch Virol 147 419–428 Occurrence Handle11890533 Occurrence Handle1:CAS:528:DC%2BD38Xkt1ensL0%3D Occurrence Handle10.1007/s705-002-8330-y

    Article  PubMed  CAS  Google Scholar 

  • J Chen JP Chen SA Langeveld AFLM Derks MJ Adams (2003) ArticleTitleMolecular characterization of carla- and potyviruses from Narcissus in China J Phytopathol 151 26–29 Occurrence Handle1:CAS:528:DC%2BD3sXisFartro%3D Occurrence Handle10.1046/j.1439-0434.2003.00674.x

    Article  CAS  Google Scholar 

  • M Chiba JC Reed AI Prokhnevsky EJ Chapman M Mawassi EV Koonin JC Carrington VV Dolja (2006) ArticleTitleDiverse suppressors of RNA silencing enhance agroinfection by a viral replicon Virology 346 7–14 Occurrence Handle16300814 Occurrence Handle1:CAS:528:DC%2BD28XhsFKrurc%3D Occurrence Handle10.1016/j.virol.2005.09.068

    Article  PubMed  CAS  Google Scholar 

  • Foster GD (1998) Carlavirus isolation and RNA extraction. In: Methods Mol Biol 81: Plant virology protocols: From virus isolation to transgenic resistance. pp 145–150

  • NO Kalinina DV Rakitina AG Solovyev J Schiemann SY Morozov (2002) ArticleTitleRNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block Virology 296 321–329 Occurrence Handle12069530 Occurrence Handle1:CAS:528:DC%2BD38XksFOgtLk%3D Occurrence Handle10.1006/viro.2001.1328

    Article  PubMed  CAS  Google Scholar 

  • S Kumar K Tamura M Nei (2004) ArticleTitleMEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment Brief Bioinform 5 150–163 Occurrence Handle15260895 Occurrence Handle1:CAS:528:DC%2BD2cXntFGqu7s%3D Occurrence Handle10.1093/bib/5.2.150

    Article  PubMed  CAS  Google Scholar 

  • SY Morozov AG Solovyev (2003) ArticleTitleTriple gene block: modular design of a multifunctional machine for plant virus movement J Gen Virol 84 1351–1366 Occurrence Handle12771402 Occurrence Handle1:CAS:528:DC%2BD3sXks1Gmtrs%3D Occurrence Handle10.1099/vir.0.18922-0

    Article  PubMed  CAS  Google Scholar 

  • H Perlmann P Perlmann (1994) Enzyme-linked immunosorbent assay Cell biology: A laboratory handbook Academic Press San Diego, CA 322–328

    Google Scholar 

  • P Rice I Longden A Bleasby (2000) ArticleTitleEMBOSS: The European Molecular Biology Open Software Suite Trends Genet 16 276–277 Occurrence Handle10827456 Occurrence Handle1:CAS:528:DC%2BD3cXjvVygsbs%3D Occurrence Handle10.1016/S0168-9525(00)02024-2

    Article  PubMed  CAS  Google Scholar 

  • IM Roberts DJ Robinson BD Harrison (1984) ArticleTitleSerological relationships and genome homologies among geminiviruses J Gen Virol 65 1723–1730

    Google Scholar 

  • MN Rozanov EV Koonin AE Gorbalenya (1992) ArticleTitleConservation of the putative methyltransferase domain: a hallmark of the Sindbis-like supergroup of positive-strand RNA viruses J Gen Virol 73 2129–2134 Occurrence Handle1645151 Occurrence Handle1:CAS:528:DyaK38XlsVKrtbw%3D Occurrence Handle10.1099/0022-1317-73-8-2129

    Article  PubMed  CAS  Google Scholar 

  • J Sambrook EF Fritsch T Maniatis (1989) Molecular cloning: a laboratory manual EditionNumber2 Cold Spring Harbor Laboratory New York

    Google Scholar 

  • Zheng HY, Chen J, Adams MJ, Chen JP (2006) Complete nucleotide sequence and affinities of the genomic RNA of Narcissus common latent virus (genus Carlavirus). Arch Virol 151: in press (DOI 10.1007/s00705-006-0727-x)

  • HY Zheng J Chen MF Zhao L Lin JP Chen JF Antoniw MJ Adams (2003) ArticleTitleOccurrence and sequences of Lily mottle virus and Lily symptomless virus in plants grown from imported bulbs in Zhejiang province, China Arch Virol 148 2419–2428 Occurrence Handle14648295 Occurrence Handle1:CAS:528:DC%2BD3sXptleksrk%3D Occurrence Handle10.1007/s00705-003-0207-5

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. China

    J. Chen, Y.-H. Shi, Y.-W. Lu & J.-P. Chen

  2. College of Life Science, Zhejiang University, Hangzhou, P.R. China

    Y.-H. Shi & Y.-W. Lu

  3. Wheat Pathogenesis Programme, Plant-Pathogen Interactions Division, Rothamsted Research, Harpenden, Herts, U.K.

    M. J. Adams

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  1. J. Chen
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  2. Y.-H. Shi
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  3. Y.-W. Lu
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  4. M. J. Adams
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  5. J.-P. Chen
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Chen, J., Shi, YH., Lu, YW. et al. Narcissus symptomless virus: a new carlavirus of daffodils. Arch Virol 151, 2261–2267 (2006). https://doi.org/10.1007/s00705-006-0801-4

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  • DOI: https://doi.org/10.1007/s00705-006-0801-4

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