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Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida

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Abstract

The crude root-peel extract of Flemingia vestita, genistein and praziquantel were tested against some selected glycolytic enzymes—hexokinase (HK), phosphofructokinase (PFK), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate kinase (PK), lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and malic enzyme (ME)—of the fowl tape worm, Raillietina echinobothrida. Following exposure to the various treatments, the activities of HK, PFK, PEPCK and LDH increased by 33–39%, 41–125%, 44–49% and 55–67%, respectively, and that of PK decreased by 14–26% in the parasite at the time of paralysis. The MDH and ME activities of the tissue homogenate were also found to be higher by 22–43% and 28–59%, respectively, in the treatments. However, whereas the activity of both cytosolic and mitochondrial MDH increased by 33–58% and 43–73%, respectively, the cytosolic ME activity showed an increase of 33–39%, and there was no significant enhancement in the mitochondrial ME activity. Histochemically, the enhancement in the activities of HK, LDH and MDH was clearly discernible. The enhanced glycolytic activity seems to be a function of anthelmintic stress caused by the phytochemicals.

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References

  • Ahmad M, Nizami WA (1987) In vitro effects of mebendazole on the carbohydrate metabolism of Avitellina lahorea (Cestoda). J Helminthol 61:247–252

    CAS  PubMed  Google Scholar 

  • Arme C, Pappas PW (1983) Biology of the Eucestoda, vol 2. Academic Press, New York

  • Banu MJ, Nellaiappan K, Dhandayuthapani S (1989) Lactate dehydrogenase from adult Setaria digitata (Nematoda: Onchocercidae). Vet Parasitol 32:311–323

    Article  CAS  PubMed  Google Scholar 

  • Banu MJ, Nellaiappan K, Dhandayuthapani S (1992) Mitochondrial malate dehydrogenase and malic enzyme of a filarial worm Setaria digitata: some properties and effects of drugs and herbal extracts. Jpn J Med Sci Biol 45:137–150

    CAS  PubMed  Google Scholar 

  • Beis I, Theophilidis G (1982) Phosphofructokinase in the plerocercoids of Schistocephalus solidus (Cestoda: Pseudophyllidea). Int J Parasitol 12:389-393

    Article  CAS  PubMed  Google Scholar 

  • Bergmeyer HU (1974) Methods of enzymatic analysis. Springer, Berlin Heidelberg New York

  • Bryant C, Flockhart HA (1986) Biochemical strain variation in parasitic helminths. Adv Parasitol 25:276–319

    Google Scholar 

  • Bücher T, Pfleiderer G (1955) Pyruvate kinase from muscle. In: Colowick SP, Kalplan NO (eds), Methods in enzymology, vol 1, Academic Press, New York, p 435

  • Buckwitz D, Jacobasch G, Gerth C, Holzhutter HG, Themm R (1988) A kinetic model of phosphofructokinase from Plasmodium berghei. Influence of ATP and fructose 6-phosphate. Mol Biochem Parasitol 27:225–232

    Article  CAS  PubMed  Google Scholar 

  • Burke WF, Gracy RW, Harris BG (1972) Studies on enzymes from parasitic helminths. III. Purification and properties of lactate dehydrogenase from tapeworm, Hymenolepis diminuta. Comp Biochem Physiol 43B:345–359

    Google Scholar 

  • Croxton FE, Cowden DJ, Klein S (1982) Applied general statistics, 3rd edn. Prentice Hall, New Delhi

  • Das JR, Saha N, Ratha BK (1991) Tissue distribution and sub-cellular localization of glutamate dehydrogenase in a freshwater air breathing teleost, Heteropneustes fossilis. Biochem Syst Ecol 19:207–212

    Article  CAS  Google Scholar 

  • Delabre-Defayolle I, Sarciron ME, Audin P, Gabrion C, Duriez T, Paris J, Petavy AF (1989) Echinococcus multilocularis metacestodes: biochemical and ultrastructural investigations on the effect of isatin (2-3 indoline dione) in vivo. J Antimicrob Chemother 23:237–245

    CAS  PubMed  Google Scholar 

  • Fioravanti CF, Saz HJ (1980) Energy metabolism of adult Hymenolepis diminuta. In: Arai HP (ed) Biology of the tapeworm Hymenolepis diminuta. Academic Press, New York, pp 463–504

  • Fukumoto S (1985) Pyruvate kinase isoenzymes and phosphoenolpyruvate carboxykinase during development of Spirometra erinacei. Yonago Acta Med 28:89–105

    CAS  Google Scholar 

  • Hussain H, Shukla OP, Ghatak S, Kaushal NA (1990) Enzymes of PEP-succinate pathway in Setaria cervi and effect of anthelmintic drugs. Indian J Exp Biol 28:871–875

    CAS  PubMed  Google Scholar 

  • Kar PK, Tandon V, Saha N (2002) Anthelmintic efficacy of Flemingia vestita: genistein-induced effect on the activity of nitric oxide synthase and nitric oxide in the trematode parasite, Fasciolopsis buski. Parasitol Int 51:249–257

    Article  PubMed  Google Scholar 

  • Kun E, Volfin P (1966) Tissue specificity of malate dehydrogenase isozymes. Kinetic discrimination by oxaloacetate and its mono and difluoro analogues. Biochem Biophys Res Commun 22:187

    CAS  PubMed  Google Scholar 

  • Lakhdar-Ghazal F, Blonski C, Willson M, Michels P, Perie J (2002) Glycolysis and proteases as targets for the design of new anti-trypanosome drugs. Curr Top Med Chem 2:439–456

    CAS  PubMed  Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin-phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Mommsen TP, Patrick JW, Moon TW (1985) Gluconeogenesis in hepatocytes and kidney of Atlantic salmon. Mol Physiol 8:89–100

    CAS  Google Scholar 

  • Olson JE, Anfinsen CB (1952) The crystallization and characterization of L-glutamic acid dehydrogenase. J Biol Chem 197:67–79

    CAS  PubMed  Google Scholar 

  • Omar MS, Raoof AM, Al-Amari OM (1996) Onchocerca fasciata: enzyme histochemistry and tissue distribution of various dehydrogenases in the adult female worm. Parasitol Res 82:32–37

    Article  CAS  PubMed  Google Scholar 

  • Pal P, Tandon V (1998a) Anthelmintic efficacy of Flemingia vestita (Fabaceae): genistein-induced alterations in the ultrastructure of the tegument in the cestode, Raillietina echinobothrida. J Parasitic Dis 22:104–109

    Google Scholar 

  • Pal P, Tandon V (1998b) Anthelmintic efficacy of Flemingia vestita (Fabaceae): genistein-induced alterations in the activity of tegumental enzymes in the cestode, Raillietina echinobothrida. Parasitol Int 47:233–243

    Article  Google Scholar 

  • Pal P, Tandon V (1998c) Anthelmintic efficacy of Flemingia vestita (Fabaceae): genistein-induced alterations in the esterase activity in the cestode, Raillietina echinobothrida. J Biosci 23:25–31

    Google Scholar 

  • Pearse AGE (1972) Histochemistry: theoretical and applied, 3rd edn, vol 2. Churchill Livingstone, Edinburgh

  • Probert AJ, Sharma RK, Singh K, Saxena R (1981) The effect of five fasciolicides on malate dehydrogenase activity and mortality of Fasciola gigantica, Fasciolopsis buski and Paramphistomum explanatum. J Helminthol 55:115–122

    CAS  PubMed  Google Scholar 

  • Rahman MS, Mettrick DF (1982) Carbohydrate metabolism in Hymenolepis microstoma (Cestoda). Int J Parasitol 12:155–162

    Article  CAS  PubMed  Google Scholar 

  • Rao HSP, Reddy KS (1991) Isoflavones from Flemingia vestita. Fitoterapia 63:458

    Google Scholar 

  • Reynolds CH (1980) Phosphoenolpyruvate carboxykinase from the rat and from the tapeworm, Hymenolepis diminuta. Comp Biochem Physiol 65B:481–487

    CAS  Google Scholar 

  • Roy B, Tandon V (1996) Effect of root-tuber extract of Flemingia vestita, a leguminous plant, on Artyfechinostomum sufrartyfex and Fasciolopsis buski: an electron microscopy study. Parasitol Res 82:248–252

    Article  CAS  PubMed  Google Scholar 

  • Sharma RK, Singh K, Saxena R, Saxena KK (1986) Effect of some anthelmintics on malate dehydrogenase activity and mortality in two avian nematodes Ascaridia galli and Heterakis gallinae. Angew Parasitol 27:175–180

    CAS  PubMed  Google Scholar 

  • Smyth JD, McManus DP (1989) The physiology and biochemistry of cestodes. Cambridge University Press, Cambridge

  • Srinivasan NG, Wariso BA, Kulkarni G, Rao GS, Harris BG (1988) Phosphofructokinase from Dirofilaria immitis. Stimulation of activity by phosphorylation with cyclic AMP-dependent protein kinase. J Biol Chem 263:3482–3485

    CAS  PubMed  Google Scholar 

  • Tandon V, Pal P, Roy B, Rao HSP, Reddy KS (1997) In vitro anthelmintic activity of root tuber extract of Flemingia vestita, an indigenous plant in Shillong, India. Parasitol Res 83:492–498

    Article  CAS  PubMed  Google Scholar 

  • Tandon V, Pal P, Saha N (1998) Anthelmintic efficacy of Flemingia vestita (Fabaceae): genistein-induced alterations in the free amino acid pool of the cestode, Raillietina echinobothrida. J Parasitic Dis 22:110–115

    Google Scholar 

  • Tandon V, Das B, Saha N (2003) Anthelmintic efficacy of Flemingia vestita (Fabaceae): effect of genistein on glycogen metabolism in the cestode, Raillietina echinobothrida. Parasitol Int 52:179–183

    Article  CAS  PubMed  Google Scholar 

  • Tielens AG, Van der Meer P, Van den Heuvel JM, Van den Bergh SG (1991) The enigmatic presence of all gluconeogenic enzymes in Schistosoma mansoni adults. Parasitology 102:267–276

    CAS  PubMed  Google Scholar 

  • Veerakumari L, Munuswamy N (2000) In vitro effect of some anthelmintics on lactate dehydrogenase activity of Cotylophoron cotylophorum (Digenea: Paramphistomidae). Vet Parasitol 91:129–140

    Article  CAS  PubMed  Google Scholar 

  • Vorhaben JE, Campbell JW (1972) Glutamine synthetase: a mitochondrial enzyme in uricotelic species. J Biol Chem 247:2763–2767

    CAS  PubMed  Google Scholar 

  • Vykhrestyuk NP, Klochkova VI (1984) Hexokinase in the trematode Calicophoron ijimai, the cestode Bothriocephalus scorpii and the turbellarian, Penecurva sibirica (in russian). Parazity zhivotnykh i rastenii. Academiya Nauk SSSR, Dal’nevostochnyi Nauchnyi Tsentr, Biologo-Poch vennyi Institut, Vladivostok, pp 82–86

  • Vykhrestyuk NP, Burenina EA, Yarygina GV (1984) Fermentation and properties of some enzymes of carbohydrate metabolism in the trematode, Calicophoron ijimai. Mol Biochem Parasitol 13:29–38

    Article  CAS  PubMed  Google Scholar 

  • Wani JH, Srivastava VM (1994) Effect of cations and anthelmintics on enzymes of respiratory chains of the cestode Hymenolepis diminuta. Biochem Mol Biol Int 34:239–250

    CAS  PubMed  Google Scholar 

  • Xiao SH, Feng JJ, Guo HF, Jiao PY, Yao MY, Jiao W (1993) Effects of mebendazole, albendazole, and praziquantel on succinate dehydrogenase, fumarate reductase, and malate dehydrogenase in Echinococcus granulosus cysts harbored in mice. Zhongguo Yao Li Xue Bao 14:151–154

    CAS  PubMed  Google Scholar 

  • Xiao SH, You JQ, Guo HF, Jiao PY, Mei JY, Yao MY, Feng Z (1998) Effect of artemether on glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and pyruvate kinase of Schistosoma japonicum harbored in mice. Zhongguo Yao Li Xue Bao 19:279-281

    CAS  PubMed  Google Scholar 

  • Xiao SH, You JQ, Guo HF, Mei JY, Jiao PY, Yao MY, Zhuang ZN, Feng Z (1999) Effect of artemether on phosphorylase, lactate dehydrogenase, adenosine triphosphatase, and glucosephosphate dehydrogenase of Schistosoma japonicum harbored in mice. Zhongguo Yao Li Xue Bao 20:750–754

    CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by the DRS-III programme of the UGC to the Department of Zoology, NEHU, Shillong. We thank the Coordinator of the Bio-informatics Centre, NEHU, for allowing access to the Centre’s facilities.

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Das, B., Tandon, V. & Saha, N. Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida . Parasitol Res 93, 253–261 (2004). https://doi.org/10.1007/s00436-004-1122-8

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