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Melanosome uptake is associated with the proliferation and differentiation of keratinocytes

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Abstract

Melanosomes are synthesized in melanocytes and transferred to neighboring keratinocytes. However, the associations of melanosome uptake with the proliferation and differentiation of keratinocytes are not fully understood. We examined the associations of melanosome uptake with keratinocyte differentiation and proliferation. SV40T-transformed human epidermal keratinocytes (SV-HEKs) were treated with isolated melanosomes. The effects of melanosome uptake on the proliferation and differentiation of the keratinocytes were analyzed by Western blotting and flow cytometry. The relationship between melanosome uptake and keratinocyte differentiation status was verified by determining the melanin content in the cells. Melanosomes reduced the proliferation of SV-HEKs in a dose-dependent manner, but did not induce differentiation. Melanosome uptake was higher in differentiating keratinocytes compared to non-differentiating keratinocytes, and inhibited significantly by PAR-2 inhibitor. Melanosomes inhibit keratinocyte proliferation. Moreover, melanosome uptake is influenced by keratinocyte differentiation status, being highest in mid-stage differentiating keratinocytes in a PAR-2 dependent manner.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A2A10038685).

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Chungnam National University, 266 Munhwa-ro, Daejeon, 301-747, Korea

    Hye-In Choi & Young Ho Lee

  2. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea

    Kyung-Cheol Sohn, Dong-Kyun Hong, Young Lee, Chang Deok Kim & Jeung-Hoon Lee

  3. Department of Dermatology, School of Medicine, Gyeongsang National University, Jinju, Korea

    Tae-Jin Yoon

  4. Seoul Neurology Clinic, Nonsan, Korea

    Jin Woon Park

  5. Department of Plastic Surgery, Konyang University Hospital, Daejeon, Korea

    Sunggyun Jung

Authors
  1. Hye-In Choi
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  2. Kyung-Cheol Sohn
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  3. Dong-Kyun Hong
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  4. Young Lee
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  5. Chang Deok Kim
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  6. Tae-Jin Yoon
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  7. Jin Woon Park
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  8. Sunggyun Jung
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  9. Jeung-Hoon Lee
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  10. Young Ho Lee
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Corresponding author

Correspondence to Young Ho Lee.

Additional information

H.-I. Choi and K.-C. Sohn equally contributed to this work.

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Choi, HI., Sohn, KC., Hong, DK. et al. Melanosome uptake is associated with the proliferation and differentiation of keratinocytes. Arch Dermatol Res 306, 59–66 (2014). https://doi.org/10.1007/s00403-013-1422-x

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  • DOI: https://doi.org/10.1007/s00403-013-1422-x

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