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Protective role of AQP3 in UVA-induced NHSFs apoptosis via Bcl2 up-regulation

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Abstract

Aquaporin-3 (AQP3), a water/glycerol-transporting protein that facilitates water, urea, and glycerol transport, can inhibit arsenite-induced apoptosis by up-regulating Bcl-2. However, whether it has a protective role in ultraviolet A (UVA)-induced apoptosis in normal human skin fibroblasts is not known. In this study, we demonstrate that mild UVA treatment fails to induce oxidative cell stress and apoptosis in normal human skin fibroblasts (NHSFs) overexpressing AQP3. After severe UVA irradiation, there was an increase in oxidative cell stress and apoptosis when AQP3 levels decreased. We also found that silencing AQP3 sensitized NHSFs to low-dose UVA. Overexpressing AQP3 was protective against high-dose UVA-induced oxidative stress and apoptosis. Besides, we observed that Bcl-2 may be involved in UVA-induced apoptosis. Our findings suggested that the water/glycerol-transporting protein AQP3 plays a role in resistance to UVA-induced apoptosis.

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Acknowledgments

The study was supported by the National Natural Science Foundation of China (NO. 81271775, 81171520), the Research Fund for the Doctoral Program of Higher Education of China (RFDP) (20100162110035), the China Postdoctoral Science Foundation (2012M511421), the Scientific Research Fund of Hunan Provincial Science and Technology Department (2012WK3045).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China

    Hongfu Xie, Fangfen Liu, Leishan Liu, Jian Dan, Ying Luo, Yuxin Yi, Xiang Chen & Ji Li

  2. Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China

    Ji Li

  3. The State Key Laboratory of Medical Genetics, Institute of Medical Genetics, Central South University, Changsha, 410008, Hunan, China

    Ji Li

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  1. Hongfu Xie
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Correspondence to Xiang Chen or Ji Li.

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F. Liu and H. Xie are the co-first authors.

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Xie, H., Liu, F., Liu, L. et al. Protective role of AQP3 in UVA-induced NHSFs apoptosis via Bcl2 up-regulation. Arch Dermatol Res 305, 397–406 (2013). https://doi.org/10.1007/s00403-013-1324-y

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  • DOI: https://doi.org/10.1007/s00403-013-1324-y

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