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Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum

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Abstract

Maize root colonization and phosphate solubilizing activity of the fungus Penicillium rugulosum were assessed in a greenhouse trial using soil-plant microcosms. The bacterial gene hph conferring resistance to hygromicin B was introduced by electroporation in the wild-type strain IR-94MF1 of P. rugulosum and one transformant, w-T3, was selected. Maize plants were grown for 5 weeks in a P-poor soil and fertilized with a Florida apatite mineral, with Navay, an apatite rock deposit from Venezuela, or with simple superphosphate. Inoculation treatments included strain IR-94MF1, transformant w-T3 and two IR-94MF1 UV-induced mutants with enhanced (Mps++) or reduced (Mps−) in vitro mineral phosphate solubilizing activity. In the absence of P fertilization, inoculation with any P. rugulosum isolate significantly reduced the size of the total and P-solubilizing bacterial community present in maize rhizosphere. The bacterial community significantly increased in maize inoculated with IR-94MF1 and w-T3 when P was added as apatites Navay or Florida. All P. rugulosum strains were able to stimulate the growth of maize plants as indicated by 3.6 to 28.6% increases in dry matter yields. In the presence of rock phosphate, P uptake by maize plants inoculated with the two mutants Mps++ and Mps− was not always in agreement with their P-solubilizing phenotypes. Strain IR-94MF1 and transformant w-T3 increased P assimilation by the plants fertilized with Navay rock phosphate by 26 and 38%, respectively. In this treatment, w-T3 showed its highest significant maize rhizosphere colonization. With the simple superphosphate treatment, w-T3 increased P uptake in plants by 8% over the uninoculated control and also decreased significantly the community size of total bacteria, total fungi, and P-solubilizing fungi in the rhizosphere.

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References

  1. Anonymous (1994) Grilles de référence en fertilisation. Conseil des productions végétales du Québec. Québec, Canada

  2. Beever RE, Burns DJW (1980) Phosphorus uptake, storage and utilization by fungi. Adv Bot Res 8:127–219

    Article  CAS  Google Scholar 

  3. Bej AK, Perlin M, Atlas RM (1991) Effect of introducing genetically engineered microorganisms on soil microbial community diversity. FEMS Microbiol Ecol 86:169–176

    Article  Google Scholar 

  4. Berthelin JCL, Laheurte F, DeGiudici P (1991) Some considerations on the relations between phosphate solubilizing rhizobacteria and their effect on seedlings and plants growth related to phosphate mobilization. In: The Second International Workshop on Plant Growth-Promoting Rhizobacteria, Oct 14–19, 1990, Interlaken, Switzerland, Plant growth-promoting rhizobacteria progress and prospects, Bulletin SROP, pp 359–364

  5. Chabot R, Antoun H, Cescas MP (1996) Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar. phaseoli. Plant Soil 184:311–321

    Article  CAS  Google Scholar 

  6. Durand N, Reymond P, Fèvre M (1991) Transformation of Penicillium roqueforti to phleomycin and to hygromycin B-resistance. Curr Genet 19:149–153

    Article  CAS  Google Scholar 

  7. Goldman GH, Montagu MV, Herrera-Estrella A (1990) Transformation of Trichoderma harzianum by high-voltage electric pulse. Curr Genet 17:169–174

    Article  CAS  Google Scholar 

  8. Kucey RMN, Janzen HH, Leggett ME (1989) Microbially mediated increases in plant-available phosphorus. Adv Agron 42:199–228

    Article  CAS  Google Scholar 

  9. Kucey RMN, Leggett ME (1989) Increased yields and phosphorus uptake by westar canola Brassica napus L.) inoculated with a phosphate-solubilizing isolate of Penicillium bilaji. Can J Soil Sei 69:425–432

    Google Scholar 

  10. Lima JA, Nahas E, Gomes AC (1996) Microbial populations and activities in sewage sludge and phosphate fertilizer-amended soil. Appl Soil Ecol 4:75–82

    Article  Google Scholar 

  11. Lynch JM (ed) (1990) The Rhizosphere. Ecological and Applied Microbiology. John Wiley & Sons Ltd, West Sussex, UK

    Google Scholar 

  12. Mehlich A (1984) Mehlich 3 soil test extractant: a modification of Mehlich 2 extractant. Commun Soil Sei Plant Anal 15:1409–1416

    Article  CAS  Google Scholar 

  13. Miethling R, Wieland G, Backhaus H, Tebbe CC (2000) Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33. Microb Ecol 41:43–56

    Google Scholar 

  14. Migheli Q, Herrera-Estrella A, Avantaneo M, Gullino ML (1994) Fate of transformed Trichoderma harzianum in the phylloplane of tomato plants. Mol Ecol 3:153–159

    Article  Google Scholar 

  15. Migheli Q, Friard O, Del Tedesco D, Musso MR, Gullino ML (1996) Stability of transformed antagonistic Fusarium oxysporum strains in vitro and in soil microcosms. Mol Ecol 5:641–649

    Article  CAS  Google Scholar 

  16. Möller EM, Bahnweg G, Sandermann H, Geiger H (1992) A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 20:6115–6116

    Article  PubMed  Google Scholar 

  17. Page AL, Miller RH, Keeney DR (eds) (1982) Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties. Agronomy Series No 9, 2nd ed. Am Soc Agron, Soil Sei Soc Am, Madison WI

    Google Scholar 

  18. Reyes I, Bernier L, Simard RR, Tanguay P, Antoun H. (1998) Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants. FEMS Microbiol Ecol 28:291–295

    Google Scholar 

  19. Reyes I, Bernier L, Simard RR, Antoun H (1998) Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microbiol Ecol 28:281–290

    Google Scholar 

  20. Reyes I, Baziramakenga R, Bernier L, Antoun H (2001). Solubilization of phosphate rocks and minerals by a wild-type strain and two UV-induced mutants of Penicillium rugulosum. Soil Biol Biochem 33:1741–1746.

    Article  CAS  Google Scholar 

  21. Richardson AE (1994) Soil microorganisms and phosphorus availability. In: CE Pankhurst, BM Doube, VVSR Gupts, PR Grace (eds) Soil Biota, Management in Sustainable Farming Systems. CSIRO Australia, Victoria, pp 50–62

    Google Scholar 

  22. Salih HM, Yahya AI, Abdul RA, Munam BH (1989) Availability of phosphorus in a calcareous soil treated with rock phosphate or superphosphate as affected by phosphate-dissolving fungi. Plant Soil 120:181–185

    Article  CAS  Google Scholar 

  23. Sambrook J, Fritsch EE, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  24. Sarathchandra SU, Lee A, Perrott KW, Rajan SSS, Oliver EHA (1993) Effect of phosphate fertilizer applications on microorganisms in pastoral soil. Aust J Soil Res 31:299–309

    Article  CAS  Google Scholar 

  25. SAS, Institute Inc. (1990) SAS Procedures guide, Version 6 edition, SAS Institute Inc., Cary, NC, pp 705

    Google Scholar 

  26. Skatrud PL, Queener SW, Carr LG, Fisher DL (1987) Efficient integrative transformation of Cephalosporium acremonium. Curr Genet 12:337–348

    Article  PubMed  CAS  Google Scholar 

  27. Tandon HLS, Cescas MP, Tyner EH (1968) An acid-free vanadate-molybdate reagent for the determination of total phosphorus in soils. Soil Sei Soc Amer Proc 32:48–51

    CAS  Google Scholar 

  28. Tiedje JM, Colwell RK, Grossman YL, Hodson RE, Lenski RE (1989) The planned introduction of genetically engineered organisms: ecological considerations and recommendations. Ecology 70:298–315

    Article  Google Scholar 

  29. Vanlauwe B, Nwoke OC, Diels J. Sanginga N, Caarsky RJ, Deckers J, Merckx R (2000) Utilization of rock phosphate by crops on a representative toposequence in the Northern Guinea savanna zone of Nigeria: response by Mucuna prunes, Lablab purpureus and maize. Soil Biol Biochem 32:2063–2077

    Article  CAS  Google Scholar 

  30. Whitelaw MA, Harden TJ, Bender, G.L. (1997) Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31:655–665

    Article  Google Scholar 

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Authors and Affiliations

  1. Département des Sols et de Génie Agro-alimentaire, Faculté des Sciences de l’Agriculture et de l’Alimentation, Pavillon Charles-Eugène-Marchand, Université Laval, G1K 7P4, Québec, Canada

    I. Reyes & H. Antoun

  2. Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Pavillon Charles-Eugène-Marchand, Université Laval, G1K 7P4, Québec, Canada

    L. Bernier

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  1. I. Reyes
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  2. L. Bernier
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  3. H. Antoun
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Correspondence to H. Antoun.

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Online publication: 20 May 2002

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Reyes, I., Bernier, L. & Antoun, H. Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum . Microb Ecol 44, 39–48 (2002). https://doi.org/10.1007/s00248-002-1001-8

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  • DOI: https://doi.org/10.1007/s00248-002-1001-8

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