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Independent Origins of Subgroup Bl+B2 and Subgroup B3Metallo-β-Lactamases

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Abstract

The metallo-β-lactamases constitute Class B in the Ambler classification of β-lactamases and are divided into three subclasses: Bl, B2, and B3. Bayesian phylogenies of the Subclass B1+B2 and Subclass B3 metallo-β-lactamases and their homologs show that the β-lactam-hydrolyzing function evolved independently within each group. In Subclass B1+B2 that function evolved about 1 billion years ago, and in Subclass B3 it evolved before the divergence of the Gram-positive and Gram-negative eubacteria, about 2 billion years ago. These results lend additional support to the proposal that the metallo-β-lactamases should be divided into two distinct classes.

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Acknowledgments

This study was supported by Grant GM60761 from the National Institutes of Health. We are grateful to Reviewer 1 for insightful comments on an earlier version of this paper.

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  1. Biology Department, University of Rochester, Rochester, NY, 14627, USA

    Barry G. Hall, Stephen J. Salipante & Miriam Barlow

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  1. Barry G. Hall
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  2. Stephen J. Salipante
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  3. Miriam Barlow
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Correspondence to Barry G. Hall.

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Hall, B.G., Salipante, S.J. & Barlow, M. Independent Origins of Subgroup Bl+B2 and Subgroup B3Metallo-β-Lactamases. J Mol Evol 59, 133–141 (2004). https://doi.org/10.1007/s00239-003-2572-9

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  • DOI: https://doi.org/10.1007/s00239-003-2572-9

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