Skip to main content
SpringerLink
Log in

Properties of the strainsEnterococcus haemoperoxidus andE. moraviensis, new species among enterococci

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Antibiotic susceptibility or resistance, urease activity, detection of the structural genes for bacteriocin production, bacteriocin activity as well as sensitivity of the isolates to enterocins (Ent) A and M were determined in 23 isolates of new speciesEnterococcus haemoperoxidus andE. moraviensis. The majority of the strains were antibiotic sensitive and exhibited low urease activity (<10 nkat/mL). The most frequently detected genes for Ent wereentA andentP. However, only the strain 466 ofE. haemoperoxidus produced an antibacterial substance with inhibitory activity against 21 G+ indicators. It was partially purified reaching an activity of up to 12 800 AU/mL. This bacteriocin active strain also possessed the genes for EntA and EntP. The other strains did not inhibit the indicator strains. The substance produced by the 466 strain was active even after a 5-months storage at +4 and −20°C. This substance has proteolytic and hydrophilic character, pH optimum of bacteriocin production by this strain being between 4 and 7. WhileE. moraviensis strains showed sensitivity to EntA (produced byE. faecium EK13) and to EntM (produced byE. faecium AL41),E. haemoperoxidus strains were sensitive to EntA (except strain 382) but less sensitive to the treatment by EntM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

E.h. :

E. haemoperoxidus

Ent:

enterocin(s)

Amp:

ampicillin

Kan:

kanamycin

Tet:

tetracycline

E.m. :

E. moraviensis

PPS:

partially purified substance

Cap:

chloramphenicol

Nov:

novobiocin

Van:

vancomycin

Ery:

erythromycin

Rif:

rifampicin

References

  • Aymerich T., Holo H., Havarstein L.S., Hugas M., Garriga M., Nes I.F.: Biochemical and genetic characterization of enterocin A fromEnterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins.Appl.Environ.Microbiol. 62, 1676–1682 (1996).

    PubMed  CAS  Google Scholar 

  • Becquet P.: EU assessment of enterococci as feed additives.Internat.J.Food Microbiol. 88, 247–254 (2003).

    Article  Google Scholar 

  • Casaus P., Nilsen T., Cintas L.M., Nes I.F., Hernandez P.E., Holo H.: Enterocin B, a new bacteriocin fromEnterococcus faecium T136 which can act synergistically with enterocin A.Microbiology 143, 2287–2294 (1997).

    PubMed  CAS  Google Scholar 

  • Cintas L.M., Casaus P., Havarstain L.V., Hernandez P.E., Nes I.F.: Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin fromEnterococcus faecium P13 with abroad antimicrobial spectrum.Appl.Environ.Microbiol. 63, 4321–4330 (1997).

    PubMed  CAS  Google Scholar 

  • Cintas L.M., Casaus P., Holo H., Hernandez P.E., Nes I.F., Havarstain L.S.: Enterocins L50A and L50B, two novel bacteriocins fromEnterococcus faecium L50 are related to staphylococcal haemolysins.J.Bacteriol. 180, 1988–1994 (1998).

    PubMed  CAS  Google Scholar 

  • Cook A.R.: Urease activity in the rumen of sheep and the isolation of ureolytic bacteria.J.Gen.Microbiol. 92, 32–48 (1976).

    PubMed  CAS  Google Scholar 

  • Cuozzo S., Calvez S., Prévost H., Drider D.: Improvement of enterocin P purification process.Folia Microbiol. 51, 401–405 (2006).

    Article  CAS  Google Scholar 

  • Daněk P., Svozil B., Kumprecht I., Přikryl J., Koželuhová K.: The influence of application of the microbioticStreptococcus faecium M74 on the intestinal microflora in lambs until weaning. (In Czech)Czech J.Anim.Sci. 30, 157–164 (1985).

    Google Scholar 

  • De Vuyst L., Callewaert R., Pot B.: Characterization and antagonistic activity ofLactobacillus amylovorus DCE471 and large scale isolation of its bacteriocin amylovorin L471.Syst.Appl.Microbiol. 19, 9–20 (1996).

    Google Scholar 

  • Devriese L.A., Ceussens K., Haesebrouck F.: Characteristics ofEnterococcus caecorum strains from the intestines of different animal species.Lett.Appl.Microbiol. 12, 137–139 (1991).

    Article  Google Scholar 

  • Drahovská H., Slobodníková L., Kocínová D., Seman M., Končeková R., Trupl J., Turňa J.: Antibiotic resistance and virulence factors among clinical and food enterococci isolated in Slovakia.Folia Microbiol. 49, 763–768 (2004).

    Article  Google Scholar 

  • Facklam R., Sahm R., Teixiera L.M.:Enterococcus, pp. 297–305 inManual of Clinical Microbiology, 7th ed. (P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, R.H. Yolken, Eds). American Society for Microbiology, Washington (DC) 1999.

    Google Scholar 

  • Filipová M., Bujdáková H., Drahovská H., Lišková A., Hanzen J.: Occurrence of aminoglycoside-modifying-enzyme genesaac(6)—aph(2′), aph(3′), ant(4′) andant(6) in clinical isolates ofEnterococcus faecalis resistant to high-level of gentamicin and amikacin.Folia Microbiol. 51, 57–61 (2006).

    Article  Google Scholar 

  • Foulquié-Moreno M.R.: Microbiological and technological characterization of bacteriocin-producing enterococci.PhD Thesis, pp. 33–55. Vrije Universiteit Brussel (Belgium) 2002.

    Google Scholar 

  • Foulquié-Moreno M.R., Leisner J.J., Tee L.K., Ley C., Radu S., Rusul G., Vancanneyt M., De Vuyst L.: Characterization of two bacteriocins produced by isolates ofEnterococcus faecium from Malaysian tempeh.J.Appl.Microbiol. 92, 147–157 (2002).

    Article  Google Scholar 

  • Franz C.M.P.A., Holzapfel W.H., Stiles M.E.: Enterococci at the crossroads of food safety?Internat.J.Food Microbiol. 47, 1–24 (1999a).

    Article  CAS  Google Scholar 

  • Franz C.M.P.A., Worobo R.W., Quadri L.E.N., Schillinger U., Holzapfel W.H., Vederas J.C., Stiles M.E.: Atypical genetic locus associated with constitutive production of enterocin B byEnterococcus faecium BFE 900.Appl.Environ.Microbiol. 65, 2170–2178 (1999b).

    PubMed  CAS  Google Scholar 

  • French P., Venuti E., Fraimow H.S.:In vitro activity of novobiocin against multiresistant strains ofEnterococcus faecium.Antimicrob.Agents Chemother. 37, 2736–2739 (1993).

    PubMed  CAS  Google Scholar 

  • Fuuersted K.: Evaluation of the combination effects of ampicillin or vancomycin combined with streptomycin, gentamicin, tobramycin or netilmicin against enterococci.APMIS 97, 23–26 (1989).

    Google Scholar 

  • Girrafa G., Sisto F.: Susceptibility to vancomycin of enterococci from dairy products.Lett.Appl.Microbiol. 25, 335–338 (1997).

    Article  Google Scholar 

  • Hermann D.J., Gerding D.N.: Antimicrobial resistance among enterococci.Antimicrob.Agents Chemother. 35, 1–4 (1991).

    Google Scholar 

  • Jurkovič D., Križková R., Dušinský R., Belicová A., Sojka M., Krajčovič J., Ebringer L.: Identification and characterization of enterococci from bryndza cheese.Lett.Appl.Microbiol. 42, 553–559 (2006).

    PubMed  Google Scholar 

  • Kuhn I., Iversen A., Burman L.G., Olsson-Liljequist B., Franklin A., Finn M., Aarestrup F., Seyfarth A.M., Blanch A.R.: Epidemiology and ecology of enterococci with special reference to antibiotic resistant strains, in animals, humans and the environment. Example of an ongoing project within the European research programme.Internat.J.Antimicrob.Agents 14, 337–342 (2000).

    Article  CAS  Google Scholar 

  • Lauková A.: Biochemical and physiological properties of enterococci isolated from the rumen of calves.Czech J.Anim.Sci. 10, 857–860 (1992).

    Google Scholar 

  • Lauková A., Czikková S.: Inhibition effect of enterocin CCM4231 in the rumen fluid environment.Lett.Appl.Microbiol. 26, 215–218 (1998).

    Article  PubMed  Google Scholar 

  • Lauková A., Juriš P.: Distribution and characterization ofEnterococcus species in municipal sewages.Microbios 89, 73–80 (1997).

    PubMed  Google Scholar 

  • Lauková A., Koniarová I.: Survey of urease activity in ruminal bacteria isolated from domestic and wild ruminants.Microbios 84, 7–11 (1995).

    PubMed  Google Scholar 

  • Lauková A., Kuncová M., Kmeť V.: Isolation of several conjugative plasmids of the rumen bacteriaEnterococcus faecium.Biológia (Bratislava) 45, 533–538 (1990).

    Google Scholar 

  • Lauková A., Mareková M., Javorský P.: Detection and antimicrobial spectrum of a bacteriocin-like substance produced byEnterococcus faecium CCM 4231.Lett.Appl.Microbiol. 16, 257–260 (1993).

    Article  Google Scholar 

  • Lauková A., Czikková S., Vasilková Z., Juriš P., Mareková M.: Occurrence of bacteriocin production among environmental enterococci.Lett.Appl.Microbiol. 27, 178–182 (1998a).

    Article  PubMed  Google Scholar 

  • Lauková A., Czikková S., Vasilková Z., Juriš P., Krupicer I.: Antimicrobial effect of enterocin CCM 4231 in the cattle slurry environment.Cytobios 94, 73–79 (1998b).

    PubMed  Google Scholar 

  • Lauková A., Czikková S., Laczková S., Turek P.: Use of enterocin CCM 4231 to controlListeria monocytogenes in experimentally contaminated dry fermented Hornád salami.Internat.J.Food Microbiol. 52, 115–119 (1999).

    Article  Google Scholar 

  • Lauková A., Turek P., Mareková M., Nagy J.: Use of ent M, new variant of ent P to controlListeria innocua in experimentally contaminated Gombasek sausage.Arch.Lebensmittelhyg. 54, 25–48 (2003).

    Google Scholar 

  • Lauková A., Strompfová V., Skřivanová V., Volek Z., Jindřichová E., Marounek M.: Bacteriocin-producing strain ofEnterococcus faecium EK13 with probiotic character and its application in the digestive tract of rabbits.Biologia (Bratislava) 61, 779–782 (2006).

    Article  Google Scholar 

  • Marciňáková M., Simonová M., Lauková A.: Probiotic properties ofEnterococcus faecium EF9296 strain isolated from silage.Acta Vet.Brno 73, 513–519 (2004).

    Google Scholar 

  • Marciňáková M., Simonová M., Strompfová V., Lauková A.: Occurrence of structural enterocin genes among silage enterococci.Bull.Vet.Inst.Puławy 49, 387–391 (2005).

    Google Scholar 

  • Marciňáková M., Simonová M., Strompfová V., Lauková A.: Oral application ofEnterococcus faecium strain EE3 in healthy dogs.Folia Microbiol. 51, 239–242 (2006).

    Article  Google Scholar 

  • Mareková M., Lauková A., Skaugen M., Nes I.F.: Characterization ofEnterococcus faecium AL41 strain and its bacteriocin. Conf. Proc.Prospects of Probiotics in Prevention and Therapy of Diseases of Young, Stará Lesná (Slovakia) 2000.

  • Mareková M., Lauková A., De Vuyst L., Skaugen M., Nes I.F.: Partial characterization of bacteriocins produced by environmental strainEnterococcus faecium EK13.J.Appl.Microbiol. 94, 523–530 (2003).

    Article  PubMed  Google Scholar 

  • Mego M., Májek J., Končeková R., Ebringer L., Čierniková S., Rauko P., Kováč M., Trupl J., Slezák P., Zajac V.: Intramucosal bacteria in colon cancer and their elimination by probiotic strainEnterococcus faecium M74 with organic selenium.Folia Microbiol. 50, 443–447 (2005).

    Article  CAS  Google Scholar 

  • NCCLS (National Committe for Clinical Laboratory Standards) Document M100-S11: Performance Standards for Antimicrobial Susceptibility Testing, 11th Informat. Suppl. NCCSL, Wayne (USA) 2001.

    Google Scholar 

  • Nes I.F., Holo H.: Class II antimicrobial peptides from lactic acid bacteria.Biopolymers 55, 50–61 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Sabia C., De Niederhausern S., Messi P., Manicardi G., Bondi M.: Bacteriocin-producingEnterococcus casseliflavus Im 416K1, a natural antagonist for control ofListeria monocytogenes in Italian sausages (“cacciatore”).Internat.J.Food Microbiol. 87, 173–179 (2003).

    Article  CAS  Google Scholar 

  • Sarantinopoulos P., Leroy F., Leontopoulou E., Georlaki M.D., Kalantzopoulos G., Tsakalidou E., De Vuyst L.: Bacteriocin production byEnterococcus faecium FAIR-E198 in view of its application as adjunct in Greek Feta cheese making.Internat.J.Food Microbiol. 72, 125–136 (2002).

    Article  CAS  Google Scholar 

  • Simonová M., Lauková A., Štyriak I.: Enterococci from rabbits-potential feed additive.Czech J.Anim.Sci. 50, 416–421 (2005).

    Google Scholar 

  • Strompfová V., Lauková A., Ouwehand A.C.: Lactobacilli and enterococci — potential probiotics for dogs.Folia Microbiol. 49, 203–207 (2004).

    Article  Google Scholar 

  • Strompfová V., Simonová M., Marciňáková M., Lauková A.: Distribution of enterocins among enterococci from Slovak, French and Italian fermented meat products, p. 47 inBook of Contributions of the 4th Conf. Structure and Stability of Biomacro-molecules SSB, Košice (Slovakia) 2005.

  • Švec P., Devriese L.A., Sedláček I., Baele M., Vancanneyt M., Haesebrouck F., Swings J.D., Doškař J.:Enterococcus haemoperoxidus sp.nov. andEnt. moraviensis sp.nov., isolated from water.Internat.J.Syst.Evol.Microbiol. 51, 1567–1574 (2001a).

    Google Scholar 

  • Švec P., Sedláček I., Pantůček R., Devriese L.A., Doškař J.: Evaluation of ribotyping for characterization and identification ofEnterococcus haemoperoxidus andEnt. moraviensis strains.FEMS Microbiol.Lett. 203, 23–27 (2001b).

    Article  PubMed  Google Scholar 

  • Toba T., Yoshioka E., Itoh T.: Lacticin, a bacteriocin produced byLactobacillus delbrueckii subsp.lactis.Lett.Appl.Microbiol. 12, 43–45 (1991).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Animal Physiology, Slovak Academy of Sciences, 040 01, Košice, Slovakia

    A. Lauková

  2. Czech Collection of Microorganisms, Faculty of Science, Masaryk University, 602 00, Brno, Czechia

    P. Švec, V. Strompfová, V. Štětina & I. Sedláček

Authors
  1. A. Lauková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Švec
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Strompfová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Štětina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. Sedláček
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Lauková.

Additional information

This work was supported by the Slovak-Czech/Czech-Slovak bilateral project no. 187/164/22 byMinistry of Education of both republics; part of this work was also funded by the project VEGA 2/5139/27 ofSlovak Scientific Agency.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lauková, A., Švec, P., Strompfová, V. et al. Properties of the strainsEnterococcus haemoperoxidus andE. moraviensis, new species among enterococci. Folia Microbiol 52, 273–279 (2007). https://doi.org/10.1007/BF02931309

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02931309

Keywords

Search

Navigation