Abstract
To identify contact feeding stimulants for adult cereal leaf beetles (CLB),Oulema melanopus (L.), compounds extracted from a host plant,Hordeum vulgare L. (cultivar ‘Lakeland’), were bioassayed in 3% agar. Damage to agar gel with the hydrophilic fraction was dominated by narrow channels; the hydrophobic fraction gel was dominated by bites, rashes, and shorter channels. This difference probably reflected the functional nature of compounds in each fraction. Crude extract and the hydrophobic fraction of nonhost pea seedlings,Pisum sativum L., prevented CLB feeding behavior, but the hydrophilic fraction only reduced it. Combination of barley and pea crude extracts, by weight at 1∶1 or greater, evoked a small feeding response which indicated a host-specific recognition factor in the barley.
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References
Bate-Smith, E.C. 1972. Attractants and repellents in higher animals, p. 45–56,in J.B. Harborne (ed.).Phytochemical Ecology. Academic Press, New York.
Beck, S.D. 1965. Resistance of plants to insects.Annu. Rev. Entomol 10:207–232.
Castro, T.R., Ruppel, R.F. andGomulinski, M.S. 1965. Natural history of the cereal leaf beetle in Michigan.Mich. State Univ. Agr. Sta. Q. Bull. 47:623–653.
Christie, W.W. 1973.Lipid Analysis. Pergamon Press, New York. 338 pp.
Dethier, V.G. 1947.Chemical Insect Attractants and Repellents. Blakiston, Philadelphia. 289 pp.
Fraenkel, G.S. 1959. The raison d'être of secondary plant substances.Science (N.Y.) 129:1466–1470.
Fraenkel, G.S. 1969. Evaluation of our thoughts on secondary plant substances.Entomol. Exp. Appl. 12:473–486.
Hsiao, T.H. 1972. Chemical feeding requirements of oligophagous insects, p. 225–239,in J.G. Rodriguez (ed.).Insect and Mite Nutrition. North-Holland Publishing, Amsterdam. 702 pp.
Ishikawa, S., Hirao, T., andArai, M. 1969. Chemosensory basis of host plant selection in the silkworm.Entomol. Exp. Appl. 12:544–554.
Jermy, T. 1966. Feeding inhibitors and food preference in chewing phytophagous insects.Entomol. Exp. Appl. 9:1–12.
Johnson, A.R. 1971. Extraction and purification of lipids, p. 113–136,in A.R. Johnson and J.B. Davenport (eds.).Biochemistry and Methodology of Lipids. Wiley-Interscience, New York.
Kates, M. 1957. Effects of solvents and surface-active agents on plastid phosphatase C activity.Can J. Biochem. Physiol. 35:127–142.
Kates, M., andEberhardt, F.M. 1957. Isolation and fractionation of leaf phosphatides.Can.J. Bot. 35:895–906.
Kennedy, J.S. 1965. Mechanisms of host plant selection.Ann. Appl. Biol. 56:317–322.
Panella, J.S., Webster, J.A., andZabik, M.J. 1974. Cereal leaf beetle host selection and plant resistance: Olfactometer and feeding attractant tests (Coleoptera: Chrysomelidae).J. Kan. Entomol. Soc. 37:348–357.
Schoonhoven, L.M. 1972a. Secondary plant substances and insects, p. 197–224,in V.C. Runeckles and T.C. Tso (eds.).Recent Advances in Phytochemistry, Vol. 5.Structural and Functional Aspects of Phytochemistry. Academic Press, New York.
Schoonhoven, L.M. 1972b. Some aspects of host selection and feeding in phytophagous insects, p. 557–566,in J.G. Rodriguez (ed.).Insect and Mite Nutrition. North-Holland Publishing, Amsterdam.
Sokal, Robert R., andRohlf, James F. 1969.Biometry. W.H. Freeman, San Francisco. 776 pp.
Thorsteinson, A.J. 1958. The chemotactic influence of plant constituents on feeding by phytophagous insects.Entomol. Exp. Appl. 1:23–27.
Thorsteinson, A.J. 1960. Host selection in phytophagous insects.Annu. Rev. Entomol. 5:193–218.
Verschaffelt, E. 1910. The cause determining the selection of food in some herbivorous insects.Proc. Kon. Ned. Acad. Wetensch. 13:536–542.
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Michigan Agricultural Experiment Station Journal Article No. 8227.
The use of a trade name in this paper does nit constitute an official endorsement or approval by the USDA.
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Kon, R.T., Zabik, M.J., Webster, J.A. et al. Cereal leaf beetle response to biochemicals from barley and pea seedlings. I. Crude extract, hydrophobic and hydrophilic fractions. J Chem Ecol 4, 511–522 (1978). https://doi.org/10.1007/BF00988915
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DOI: https://doi.org/10.1007/BF00988915