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Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro

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Abstract

Mature uteroferrin (Uf; MΓ = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a MΓ of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In this report we demonstrate that 37k Uf contains two N-linked oligosaccharides which are a mixture of complex and high mannose-type oligosaccharides. The complex-type glycopeptides are biantennary and a portion may be fucosylated on the GlcNac of the chitobiose core proximal to the peptide. Only a portion of the high mannose-type oligosaccharides are phosphorylated. The remainder appear to be typical Man6-4GlcNac2 oligosaccharides found on mature Uf.

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Abbreviations

Uf:

Uteroferrin

ConA:

Concanavalin A

WGA:

Wheat Germ Agglutinin

endoH:

endo-β-N-acetylglucosaminidase H

SDS:

Sodium Dodecyl Sulfate

SDS-PAGE:

polyacrylamide gel electrophoresis in the presence of SDS

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Author notes

  1. Philippa T. K. Saunders

    Present address: MRC Reproductive Biology Unit, Centre for Reproductive Biology, EH3 9EW, Edinburgh, Scotland

  2. Catherine M. Ketcham

    Present address: Division of Hematology, Oncology, Washington University School of Medicine and Biochemistry, 63110, St Louis, MO, USA

Authors and Affiliations

  1. Department of Animal Science, College of Veterinary Medicine, The University of Tennessee, P.O. Box 1071, 37901-1071, Knoxville, TN, USA

    George A. Baumbach

  2. Department of Biochemistry and Molecular Biology, University of Florida, 32610, Gainesville, FL, USA

    Philippa T. K. Saunders & Catherine M. Ketcham

  3. Department of Animal Science, University of Florida, 32610, Gainesville, FL, USA

    Fuller W. Bazer

  4. Department of Animal Science, University of Missouri, 65211, Columbia, MO, USA

    R. Michael Roberts

Authors
  1. George A. Baumbach
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  2. Philippa T. K. Saunders
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  3. Catherine M. Ketcham
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  4. Fuller W. Bazer
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  5. R. Michael Roberts
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Baumbach, G.A., Saunders, P.T.K., Ketcham, C.M. et al. Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro . Mol Cell Biochem 105, 107–117 (1991). https://doi.org/10.1007/BF00227750

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  • DOI: https://doi.org/10.1007/BF00227750

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