Abstract
A novel form of human adenylyl cyclase (ADCY7) has been discovered in the human erythroleukemia cell line (HEL). This cell line has been widely used as a model for studies of the characteristics of human platelets. Data from HEL cells suggests that ADCY7 may be the major AC form in human platelets. In the current study polymerase chain reaction (PCR) techniques coupled with use of human/rodent somatic hybrid panels and a yeast artificial chromosome (YAC) library were used to determine the chromosomal localization of the gene (adcy7) for ADCY7 enzyme. A 251-bp product from the 3′ untranslated region of human adcy7 was amplified for PCR mapping and the results localize the adcy7 gene to region 16q12–16q13 of the human genome. The AC enzyme family is characterized by the presence of 12 membrane-spanning domains in its sequences, and this chromosomal region is known to contain other genes coding for proteins characterized by 12 membrane-spanning domains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bakalyar HA, Reed RR (1990) Identification of a specialized adenylyl cyclase that may mediate odorant detection. Science 250: 1403–1406
Brüss M, Kunz J, Lingen B, Bönisch H (1993) Chromosomal mapping of the human gene for the tricyclic antidepressant-sensitive noradrenaline transporter. Hum Genet 91: 278–280
Cali JJ, Zwaagstra JC, Mons N, Cooper DMF, Krupinski J (1994) Type VIII adenylyl cyclase: a Ca2+/calmodulin-stimulated enzyme expressed in discrete regions of rat brain. J Biol Chem 269: 12190–12195
Cohen D, Chumakov I, Weissenbach J (1993) A first-generation physical map of the human genome. Nature 366: 698–701
Devor EJ, Cloninger CR, Hoffman PL, Tabakoff B (1991) A genetic study of platelet adenylate cyclase activity: evidence for a single major locus effect in fluoride-stimulated activity. Am J Hum Genet 49: 372–377
Feinstein PG, Schrader KA, Bakalyar HA, Tang W-J, Krupinski J, Gilman AG, Reed RR (1991) Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain. Proc Natl Acad Sci USA 88: 10173–10177
Gao BN, Gilman AG (1991) Cloning and expression of a widely distributed (type IV) adenylyl cyclase. Proc Natl Acad Sci USA 88: 10178–10182
Gelernter J, Kruger S, Pakstis AJ, Pacholczyk T, Sparkes RS, Kidd KK, Amara S (1993) Assignment of the norepinephrine transporter protein (NET1) locus to chromosome 16. Genomics 18: 690–692
Haber N, Stengel D, Defer N, Roeckel N, Mattei M-G, Hanoune J (1994) Chromosomal mapping of human adenylyl cyclase genes type III, type V, and type VI. Hum Genet 94: 69–73
Hellevuo K, Yoshimura M, Kao M, Hoffman PL, Cooper DMF, Tabakoff B (1993) A novel adenylyl cyclase sequence cloned from the human erythroleukemia cell line. Biochem Biophys Res Commun 192: 311–318
Ishikawa Y, Katsushika S, Chen L, Halnon NJ, Kawabe J, Homey CJ (1992) Isolation and characterization of a novel cardiac adenylylcyclase cDNA. J Biol Chem 267: 13553–13557
Iyengar R (1993) Molecular and functional diversity of mammalian Gs-stimulated adenylyl cyclase. FASEB J 7: 768–775
Krupinski J, Coussen F, Bakalyar HA, Tang W-J, Feinstein PG, Orth K, Slaughter C, Reed RR, Gilman AG (1989) Adenylyl cyclase amino acid sequence: a possible channel or transporter like structure. Science 244: 1558–1564
Krupinski J, Lehman TC, Frankenfield CD, Zwaagstra JC, Watson PA (1992) Molecular diversity in the adenylyl cyclase family. J Biol Chem 267: 24858–24862
Lincoln SE, Daly MJ, Landers ES (1991) PRIMER: a computer program for automatically selecting PCR primers, version 0. 5. MIT Center for Genome Research and Whitehead Institute for Biomedical Research, Cambridge, Mass.
Serville F, Lacombe D, Saura R, Billeaud C, Sergent MP (1993) Townes-Brocks syndrome in an infant with translocation t(5;16). Genet Counsel 4: 109–112
Spiegel AM, Weinstein LS, Shenker A (1993) Abnormalities in G protein-coupled signal transduction pathways in human disease. J Clin Invest 92: 1119–1125
Stengel D, Parma J, Gannage M-H, Roeckel N, Mattei M-G, Barouki R, Hanoune J (1992) Different chromosomal localization of two adenylyl cyclase genes expressed in human brain. Hum Genet 90: 126–130
Tabakoff B, Hoffman PL, Lee JM, Saito T, Willard B, De Leon-Jones F (1988) Differences in platelet enzyme activity between alcoholics and nonalcoholics. N Engl J Med 318: 134–139
Villacres EC, Xia Z, Bookbinder LH, Edelhoff S, Disteche CM, Storm DR (1993) Cloning, chromosomal mapping and expression of human fetal brain type I adenylyl cyclase. Genomics 16: 473–478
Wells RG, Mohandas TK, Hediger MA (1993) Localization of the Na+/glucose cotransporter gene SGLT2 to human chromosome 16 close to the centromere. Genomics 17: 787–789
Yoshimura M, Cooper DMF (1992) Cloning and expression of a Ca2+-inhibitable adenylyl cyclase from NCB-20 cells. Proc Natl Acad Sci USA 89: 6716–6720
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hellevuo, K., Berry, R., Sikela, J.M. et al. Localization of the gene for a novel human adenylyl cyclase (ADCY7) to chromosome 16. Hum Genet 95, 197–200 (1995). https://doi.org/10.1007/BF00209401
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00209401