Abstract
In maturing fruits ofPhaseolus coccineus a soluble glucosyltransferase activity occurs which converts gibberellins into their O-glucosides. The enzyme glucosylates GA3 and structurally closely related gibberellins (GA7 and GA30) to their 3-O-glucosides by transfer of glucose preferentially from UDP-glucose.
From cell suspension cultures ofLycopersicon peruvianum cytosolic glucosyltransferases were isolated which in the presence of UDP-glucose converted GA7 and GA9 to the corresponding glucosyl esters. In both cases numerous other gibberellins failed to serve as substrates. Thus, the enzymes are UDP-glucose: gibberellin glucosyltransferases of considerable substrate specificity.
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Abbreviations
- GA:
-
gibberellin
- Gle:
-
glucosyl
- ME:
-
mercaptoethanol
- UDP:
-
uridine diphosphate
- DTE:
-
dithioerythritol
- TLC:
-
thin-layer chromatography
- HPLC:
-
high performance liquid chromatography
References
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Sembdner, G., Knöfel, H.D., Schwarzkopf, E. et al. In vitro glucosylation of gibberellins. Biol Plant 27, 231–236 (1985). https://doi.org/10.1007/BF02902167
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DOI: https://doi.org/10.1007/BF02902167