Summary
By the use of a specific histochemical staining method evidence was obtained that tolerance to heavy metals in ectomycorrizal fungi is based on the presence of metallothionein-like proteins.
The implication that tolerance in these fungi should be induced by sublethal concentrations of heavy metals has been confirmed by us. Induction of metallothionein in ectomycorrizal fungi could possibly be helpful in protection of their host plants in areas polluted by heavy metals.
In comparison with biochemical methods the histochemical method is able to locate the metal tolerance and has the added advantage that it may also be applied to mycorrhizas (root and fungus).
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References
Bradley R Aet al. 1982. New Phytol. 91, 197–210.
Enger L Tet al. 1981. J. Toxicol. Environ. Health 7, 675–690.
Ernst W H O 1985 Proc. Int. Conf. on Heavy Metals in the Environment, CEP Consultants, Edinburgh, 596–599.
Fleckenstein J 1979. Mitt. dt. Bodenkdl. Ges. 25, 451–456.
Gick Cet al. 1981 Exp. Cell Res. 132, 23–30.
Hutchinson TC 1983 Int. Conf. on Heavy Metals in the Environment. CEP Consultants, Edinburgh, 17–25.
Jernelov A and Johansson K 1983 Int. Conf. on Heavy Metals in the Environment, CEP Consultants, Edinburgh, 727–741.
Lerch K 1980 Nature 284, 368–370.
Limonard T and Smits W T M 1985 Vakblad voor Biologen 65 (13/14), 62–65.
Marx D H 1969 Phytopathology 59, 153–163.
Mayer R and Lindberg S E 1985 Proc. Int. Conf. on Heavy Metals in the Environment, CEP Consultants, Edinburgh, 351–353.
Morselt A F Wet al. 1984. Arch. Toxicol. 55, 155–160.
Morselt A F W and van Straalen N M 1986 Histochemistry 84, 45–47.
Palmer J G and Hacskaylo E 1970 Physiol. Plant. 23, 1187–1197.
Palmiter R 1985 Proc. 2nd. Int. Meeting on Metallothioneins, Zürich, p. 9.
Rauser W E 1985 Auflage 1985 Proc. 2nd Int. Meeting on Metallothioneins, Zürich, p. 24.
Ulrich B 1983 Sonderheft der Mitt. 1982, Erweiterte Auflage 1983, 9–25.
Valentin V Det al. Proc. Int. Conf. on Heavy Metals in the Environment, CEP Consultants, Edinburgh, 141–143.
Zöttl, H W 1985 Experientia 41, 1104–1113.
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Morselt, A.F.W., Smits, W.T.M. & Limonard, T. Histochemical demonstration of heavy metal tolerance in ectomycorrhizal fungi. Plant Soil 96, 417–420 (1986). https://doi.org/10.1007/BF02375146
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DOI: https://doi.org/10.1007/BF02375146