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Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

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Somatic Cell and Molecular Genetics

Abstract

A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86–88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity,suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

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Authors and Affiliations

  1. Department of Ophthalmology, Baylor College of Medicine, 77030, Houston, Texas

    G. I. Liou, S. -L. Fong, Y. Li, C. Wang & C. D. B. Bridges

  2. MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Edinburgh, UK

    J. Gosden, S. Christie, D. Rout & S. Bhattacharya

  3. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas

    P. van Tuinen & D. H. Ledbetter

  4. Howard Hughes Medical Institute and Department of Microbiology & Immunology, Baylor College of Medicine, 77030, Houston, Texas

    R. G. Cook

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  1. G. I. Liou
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  2. S. -L. Fong
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Liou, G.I., Fong, S.L., Gosden, J. et al. Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization. Somat Cell Mol Genet 13, 315–323 (1987). https://doi.org/10.1007/BF01534925

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  • DOI: https://doi.org/10.1007/BF01534925

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