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The association of mannitol oxidase with a distinct organelle in the digestive gland of the terrestrial slugArion ater

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Summary

Post nuclear supernatants prepared from homogenates of the digestive gland of the slugArion ater were analysed by rate-dependent and density-dependent density gradient zonal centrifugation. Mannitol oxidase carrying structures exhibited distinct centrifugal behaviour sedimenting more slowly than mitochondria, lysosomes and peroxisomes but faster than microsomes, and banding sharply at a density of 1.15 g/ml in sucrose gradients. By combining rate and isopycnic centrifugations mannitol oxidase carrying structures were largely separated from contaminating organelles. Electron microscopic examination of such mannitol oxidase-enriched fractions revealed the predominant presence of distinct structures of tubular form. SDS PAGE analysis indicated that the major polypeptide present had a mass of ∼ 68 kDa corresponding to the major subunit previously reported for partially purified mannitol oxidase ofHelix aspersa.

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Abbreviations

ER:

endoplasmic reticulum

SDS PAGE:

sodium dodecylsulphate polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. School of Applied Sciences, University of Wolverhampton, Wulfruna Street, WV1 1SB, Wolverhampton, England

    A. T. Large & M. J. Connock

  2. Department of Pathological Sciences, Medical School, University of Manchester, Manchester

    Carolyn J. P. Jones

Authors
  1. A. T. Large
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  2. Carolyn J. P. Jones
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  3. M. J. Connock
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Large, A.T., Jones, C.J.P. & Connock, M.J. The association of mannitol oxidase with a distinct organelle in the digestive gland of the terrestrial slugArion ater . Protoplasma 175, 93–101 (1993). https://doi.org/10.1007/BF01385006

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  • DOI: https://doi.org/10.1007/BF01385006

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