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Degradation of catechin and purification and partial characterization of catechin oxygenase fromChaetomium cupreum

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Abstract

An enzyme capable of cleaving catechin was present in the mycelium ofCheatomium cupreum. Maximum synthesis of the enzyme occurred after 15 days growth. Sucrose and maltose increased enzyme synthesis among the carbon sources tested. Catechol, protocatechuic acid and phloroglucinol carboxylic acid were the intermediates of catechin degradation.Cheatomium cupreum containedmeta-cleaving enzymes for catechol and protocatechuic acid metabolism. Pyruvate was identified as an end-product. Catechin oxygenase from the mycelium ofC. cupreum was purified to homogeneity. It was optimum at pH 7.0 and 50°C and was highly specific for catechin, with a Km of 4 μm. Its molecular size was 40 kDa, as determined by gel filtration and gel electrophoresis, and it had a pI of 9.1.p-Chloromercuric benzoate, iodoacetate, N-ethylmaleimide, 2,2′-dipyridyl and EDTA markedly inhibited the enzyme activity. It was a glycoprotein.

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References

  • Adachi, K., Iwagawa, Y., Tanioka, H. & Takeda, Y. 1966 Purification and properties of homogentisate oxygenase fromPseudomonas fluorescens.Biochimica et Biophysica Acta 118, 88–97.

    Google Scholar 

  • Aoki, K., Shinke, E. & Nishira, H. 1976 Chemical composition and molecular weight of yeast tannase.Agricultural and Biological Chemistry 40, 86–91.

    Google Scholar 

  • Arunakumari, A. & Mahadevan, A. 1984 Utilization of aromatic substances byPseudomonas solanacearum.Indian Journal of Experimental Biology 22, 32–36.

    Google Scholar 

  • Barth, G., Records, R., Bunnenberg, E. & Djerassi, C. 1971 Magnetic circular dichroism studies. XII. The determination of tryptophan in proteins.Journal of American Chemical Society 93, 2545–2547.

    Google Scholar 

  • Beechy, R.B. & Ribbons, D.W. 1972 Oxygen uptake measurement.Methods in Microbiology 6b, 25–53.

    Google Scholar 

  • Cambie, R.C. 1959 The extractives ofDysoxylum spectalile, Hook. Journal of the Chemical Society 468–469.

  • Chandra, T., Madhavakrishna, W. & Nayudamma, Y. 1969 Astringency in fruits. I. Microbial degradation of catechin.Canadian Journal of Microbiology 15, 303–306.

    Google Scholar 

  • Chandrakantha, A.A., Krishnamurthy, V., Madhavakrishna, W. & Nayudamma, Y. 1973 Astringency in fruits. VI. Microbial degradation of cashew apple (Anacardium occidentale) tannin.Leather Science 20, 337–342.

    Google Scholar 

  • Dawson, R.M.C., Elliot, D.C., Elliot, W.H. & Jones, K.M. 1986 Detection of biochemical compounds. InData for Biochemical Research, eds Dawson, R.M.C., Elliot, D.C., Elliot, W.H. & Jones, K.M. p. 466 Oxford: Clarendon Press.

    Google Scholar 

  • Galiotou-Panayotou, M. & Macris, B.J. 1986 Degradation of condensed tannin byCalvatia gigantea.Applied Microbiology and Biotechnology 23, 502–506.

    Google Scholar 

  • Galiotou-Panayotou, M., Rodis, P., Macris, B.J. & Stathakos, D. 1988 Purification of a novel enzyme involved in catechin degradation byCalvatia gigantea.Applied Microbiology and Biotechnology 28, 543–545.

    Google Scholar 

  • Gauthier, J.J. & Rittenberg, S.C. 1971 Metabolism of nicotinic acid. I. Purification and properties of 2,5-dihydroxy pyridine oxygenase fromPseudomonas putida N-9.Journal of Biological Chemistry 246, 3737–3742.

    Google Scholar 

  • Hayaishi, O. 1974 Nomenclature, classification and general properties of oxygenases. InMolecular Mechanisms of Oxygen Activation, ed Hayaishi, O. pp. 6–10, New York: Academic Press.

    Google Scholar 

  • Hayaishi, O., Katagiri, M. & Rothberg, S. 1957 Studies on oxygenases. Pyrocatechase.Journal of Biological Chemistry 229, 905–920.

    Google Scholar 

  • Hegeman, G.D. 1966a Synthesis of the enzymes of the mandalate pathway byPseudomonas putida. I. Synthesis of enzymes by wild type.Journal of Bacteriology 91, 1140–1154.

    Google Scholar 

  • Hegeman, G.D. 1966b Synthesis of the enzymes of the mandalate pathway ofPseudomonas putida. II. Isolation and properties of blocked mutants.Journal of Bacteriology 91, 1155–1160.

    Google Scholar 

  • Jeffrey, A.M., Knight, M. & Evans, W.C. 1969 The bacterial metabolism of flavonoids: Hydroxylation of taxifolin.Journal of General Microbiology 56, i.

    Google Scholar 

  • Jeffrey, A.M., Knight, M. & Evans, W.C. 1972 The bacterial degradation of flavonoids.Biochemical Journal 130, 373–381.

    Google Scholar 

  • Kaneda, N., Sato, M. & Yagi, K. 1982 Analysis of dansyl amino acids by reverse phase high performance liquid chromatography.Analytical Biochemistry 127, 49–54.

    Google Scholar 

  • Lewis, J.A. & Starkey, R.L. 1968 Vegetable tannin and their decomposition and effects on decomposition of organic compounds.Soil Science 106, 241–247.

    Google Scholar 

  • Mahadevan, A., Sambandam, T. & Sivaswamy, N. 1982 Microbial degradation of phenolic substances.Indian Review of Life Sciences 2, 1–18.

    Google Scholar 

  • Mahadevan, A. & Sridhar, R. (eds) 1986Methods in Physiological Plant Pathology. Madras: Sivakami Publications.

    Google Scholar 

  • Mason, H. S. 1949 The chemistry of melanin. VI. Mechanism of the oxidation of catechol by tyrosinase.Journal of Biological Chemistry 181, 803–812.

    Google Scholar 

  • Muthukumar, G., Arunakumari, A. & Mahadevan, A. 1982 Degradation of aromatic compounds byRhizobium spp.Plant and Soil 69, 163–169.

    Google Scholar 

  • Myers, W.F. & Huang, K.Y. 1966 Separation of intermediates of the citric acid cycle and related compounds by thin layer chromatography.Analytical Biochemistry 17, 210–215.

    Google Scholar 

  • Nakai, C., Kakamiyama, H., Saeki, Y. & Nozaki, M. 1979 Nonidentical subunits of pyrocatechase fromPseudomonas arvilla C-1.Archives of Biochemistry and Biophysics 195, 12–22.

    Google Scholar 

  • Oka, T. & Simpson, F.J. 1971 Quercetinase, a dioxygenase containing copper.Biochemical and Biophysical Research Communications 43, 1–5.

    Google Scholar 

  • Peterson, G.L. 1979 Review of the Folin phenol quantitation method of Lowry, Rosebrough, Farr and Randall.Analytical Biochemistry 100, 201–220.

    Google Scholar 

  • Rajagopalan, K.V. & Handler, P. 1964 The absorption spectra of iron-flavoproteins.Journal of Biological Chemistry 239, 192–205.

    Google Scholar 

  • Reddy, C.C. & Vaidyanathan, C.S. 1975 Purification, properties and induction of a specific benzoate-4-hydroxylase fromAspergillus niger (UBC 814).Biochimica et Biophysica Acta 384, 46–57.

    Google Scholar 

  • Roux, D.G., Miller, T.H. & Maihs, E.A. 1961 An improved lead salt method for purifying black wattle tannin.Journal of American Leather Chemists' Association 56, 362–364.

    Google Scholar 

  • Sedlick, J. & Lindsay, R.H. 1968 Estimation of total, protein bound and non-protein sulphydryl groups in tissues with Ellman's reagent.Analytical Biochemistry 25, 192–205.

    Google Scholar 

  • Segrest, J.P. & Jackson, R.L. 1972 Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis on sodium dodecyl sulphate.Methods in Enzymology 28, 54–63.

    Google Scholar 

  • Simpson, R.J., Neuberger, M.R. & Linct, Y. 1976 Complete amino acid analysis of a protein from a single hydrolysate.Journal of Biological Chemistry 251, 1936–1940.

    Google Scholar 

  • Spiess, J. 1986 Carboxy terminal by modified Edman degradation. InMicrostructural Studies in Peptide and Protein Chemistry, ed Shivley, J.E. pp. 363–377. New Jersey: Humanan Publications.

    Google Scholar 

  • Sze, I.S.Y. & Dagley, A. 1984 Properties of salicylate hydrooxylase and hydroxyquinol 1,2-dioxygenase purified fromTrichosporon cutaneum.Journal of Bacteriology 159, 353–359.

    Google Scholar 

  • Updegraff, D.M. & Grant, W.D. 1975 Microbial utilization ofPinus radiata bark.Applied Microbiology 30, 722–726.

    Google Scholar 

  • Vega, R.R., Corsini, D. & LeTourneau, D. 1970 Nonvolatile organic acids produced bySclerotinia sclerotium in synthetic liquid media.Mycologia 62, 332–338.

    Google Scholar 

  • Weber, K. & Osborn, M. 1969 The reliability of molecular weight determinations by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.Journal of Biological Chemistry 244, 4406–4412.

    Google Scholar 

  • William, F. & Mahadevan, A. 1980 Degradation of aromatic compounds byXanthomonas species.Journal of Plant Diseases and Protection 87, 738–744.

    Google Scholar 

  • Yoshida, R., Hori, K., Puriware, M., Saeki, Y., Kagamiyama, H. & Nozaki, M. 1976 Nonidentical subunits of protocatechuate 3,4-dioxygenase.Biochemistry 15, 4048–4053.

    Google Scholar 

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Authors
  1. T. Sambandam
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  2. A. Mahadevan
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Additional information

T. Sambandam was and A. Mahedevan is with the Center for Advanced Studies in Botany, University of Madras, Guindy Campus, Madras-600025, India. T. Sambandam is now with the Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

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Sambandam, T., Mahadevan, A. Degradation of catechin and purification and partial characterization of catechin oxygenase fromChaetomium cupreum . World J Microbiol Biotechnol 9, 37–44 (1993). https://doi.org/10.1007/BF00656513

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  • DOI: https://doi.org/10.1007/BF00656513

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