Summary
The human thyroglobulin gene was mapped by in situ hybridization whereby a 3H-labeled recombinant plasmid DNA containing a fragment of 2.3 kilobases of human thyroglobulin gene was hybridized to human chromosome preparations. A high proportion (25%) of hybridized metaphases exhibited silver grains at the distal portion of the long arm of chromosome 8. Analysis of the grain position at this site indicated that the chromosomal localization of the human thyroglobulin gene was 8q242-8q243.
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References
Avvedimento VE, Musti AM, Obici S, Cocozza S, Di Lauro R (1984) Structural organization of the 3′ half of the rat thyroglobulin gene. Nucleic Acids Res 12:3461–3472
Bergé-Lefranc JL, Cartouzou G, Malthiery Y, Perrin F, Jarry B, Lissitzky S (1981) Cloning of four DNA fragments complementary to human thyroglobulin messenger RNA. Eur J Biochem 120:1–7
Cabrer B, Bergé-Lefranc JL, Brocas H, Cartouzou G, Pohl V, Malthiery Y (1983) Construction of a restriction map covering 95% of human thyroglobulin structural gene. Ann Endocrinol (Paris) 44:34
Camargo M, Cervenka J (1982) Patterns of DNA replication of human chromosomes. II. Replication map and replication model. Am J Hum Genet 34:757–780
Chebath J, Chabaud O, Bergé-Lefranc JL, Cartouzou G, Lissitzky S (1977) Molecular weight of the thyroglobulin messenger RNA of sheep thyroid gland. Biochem Biophys Res Commun 79:267–273
Gerhard DS, Kawasaki ES, Bancroft FC, Szabo P (1981) Localization of a unique gene by direct hybridization in situ. Proc Natl Acad Sci USA 78:3755–3759
Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439
Harper ME, Ullrich A, Saunders G (1981) Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc Natl Acad Sci USA 78:4458–4460
Lissitzky S (1984) Thyroglobulin entering into molecular biology. J Endocrinol Invest 7:65–76
Lissitzky S, Mauchamp J, Reynaud J, Rolland M (1975) The constituent polypeptide chain of porcine thyroglobulin. FEBS Lett 60:359–363
Malcolm S, Barton P, Murphy C, Ferguson-Smith MA, Bentley DL, Rabbitts TH (1982) Localization of human immunoglobulin k light variable region genes to the short arm of chromosome 2 by in situ hybridization. Proc Natl Acad Sci USA 79:4957–4961
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, New York, pp 164–166
McDonnel MW, Simon MN, Studier FW (1977) Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 110:119–146
Neel BG, Jhanwar SC, Chaganti RSK, Hayward WS (1982) Two human c-one genes are located on the long arm of chromosome 8. Proc Natl Acad Sci USA 79:7842–7846
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Szabo P, Ward DC (1982) What's new with hybridization in situ. TIBS 7:425–427
Van Ommen GJB, Arnberg AC, Baas F, Borcas H, Sterk A, Tegelaers HH, Vassart G, de Vijlder JJM (1983) The human thyroglobulin gene contains two 15–17 kb introns near its 3′-end. Nucleic Acids Res 11:2273–2285
Van Ommen GBJ, Baas F, Arnberg AC, Pearson PL, de VijlderJJM (1984) Chromosome mapping and polymorphism study of the human thyroglobulin gene. Cytogenet Cell Genet 37:562
Vassart G, Verstreken L, Dinsart C (1977) Molecular weight of thyroglobulin 33 S messenger RNA as determined by polyacrylamide gel electrophoresis in the presence of formamide. FEBS Lett 79:15–18
Zabel BU, Naylor SL, Sakaguchi AY, Bell GI, Shows TB (1983) High-resolution chromosomal localization of human genes for amylase, propiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization. Proc Natl Acad Sci USA 80:6932–6936
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Bergé-Lefranc, JL., Cartouzou, G., Mattéi, MG. et al. Localization of the thyroglobulin gene by in situ hybridization to human chromosomes. Hum Genet 69, 28–31 (1985). https://doi.org/10.1007/BF00295525
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DOI: https://doi.org/10.1007/BF00295525