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Higher-plant chloroplast and cytosolic fructose-1,6-bisphophosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence

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Abstract

Full-size cDNAs encoding the precursors of chloroplast fructose-1,6-bisphosphatase (FBP), sedoheptulose-1,7-bisphosphatase (SBP), and the small subunit of Rubisco (RbcS) from spinach were cloned. These cDNAs complete the set of homologous probes for all nuclear-encoded enzymes of the Calvin cycle from spinach (Spinacia oleracea L.). FBP enzymes not only of higher plants but also of non-photosynthetic eukaryotes are found to be unexpectedly similar to eubacterial homologues, suggesting a eubacterial origin of these eukaryotic nuclear genes. Chloroplast and cytosolic FBP isoenzymes of higher plants arose through a gene duplication event which occurred early in eukaryotic evolution. Both FBP and SBP of higher plant chloroplasts have acquired substrate specificity, i.e. have undergone functional specialization since their divergence from bifunctional FBP/SBP enzymes of free-living eubacteria.

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Abbreviations

FBP:

fructose-1,6-bisphosphatase

SBP:

sedoheptulose-1,7-bisphosphatase

FBA:

fructose-1,6-bisphosphate aldolase

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Authors and Affiliations

  1. Institut für Genetik, Technische Universität Braunschweig, Spielmannstrasse 7, D-38106, Braunschweig, Germany

    William Martin & Katrin Henze

  2. Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Königin-Luise-Strasse 12–16a, D-14195, Berlin, Germany

    Abdel-Zaher Mustafa & Claus Schnarrenberger

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  1. William Martin
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  2. Abdel-Zaher Mustafa
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  3. Katrin Henze
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  4. Claus Schnarrenberger
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Martin, W., Mustafa, AZ., Henze, K. et al. Higher-plant chloroplast and cytosolic fructose-1,6-bisphophosphatase isoenzymes: origins via duplication rather than prokaryote-eukaryote divergence. Plant Mol Biol 32, 485–491 (1996). https://doi.org/10.1007/BF00019100

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  • DOI: https://doi.org/10.1007/BF00019100

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