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Biochemical Diagnosis of Acute Aortic Damage - Diagnosis of Aortic Dissection and Traumatic Aortic Rupture Using an Immunoassay of Smooth Muscle Myosin Heavy Chain

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Advances in Critical Care Testing

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Abstract

In the setting of acute medicine, aortic dissection is a very common aortic catastrophe associated with a high mortality and morbidity rate. In the acute trauma setting, aortic rupture is an equally life-threatening event. A reliable biochemical diagnostic method for acute aortic damage would be beneficial to the critical care specialist

A novel immunoassay of smooth muscle-specific myosin heavy chain was developed. The clinical usefulness for detection of acute aortic diseases, aortic dissection and traumatic aortic rupture was investigated

Forty patients with aortic dissection were examined. The results showed significant elevations of serum smooth muscle myosin heavy chain during the first 24 h. The sensitivity of the assay was 87% within the first 12 h and 82% within the first 24 h at a cut-off level of 2.5 ng/ml. The specificity of the assay was 97%. Two patients with traumatic aortic rupture also showed significant elevations of serum smooth muscle myosin heavy chain. The immunoassay of serum smooth muscle myosin heavy chain is a rapid and reliable biochemical method in the diagnosis of acute aortic diseases, aortic dissection and traumatic aortic rupture. The potential use of the method in the critical care setting is promising.

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References

  1. LaDue JS, Wroblewski F, Karmen A (1954) Serum glutamic oxaloacetic transaminase activity in human acute transmural myocardial infarction. Science 120:497–499.

    Article  PubMed  CAS  Google Scholar 

  2. Sorensen NS (1963) Creatine phosphokinase in the diagnosis of myocardial infarction. Acta Med Scand 174:725–734.

    Article  PubMed  CAS  Google Scholar 

  3. Kibe O, Nilsson NJ (1967) Observations on the diagnostic and prognostic value of some enzyme tests in myocardial infarction. Acta Med Scand 182:597–610.

    Article  PubMed  CAS  Google Scholar 

  4. Katoh H, Sugi M, Chino S et al. (1992) Development of an immunoradiometric assay kit for ventricular myosin light chain I with monoclonal antibodies. Clin Chem 38:170–171.

    PubMed  CAS  Google Scholar 

  5. Trahern CA, Gere JB, Kurauth GH, Bigham DA (1978) Clinical assessment of serum myosin light chains in the diagnosis of acute myocardial infarction. Am J Cardiol 41:641–645.

    Article  PubMed  CAS  Google Scholar 

  6. Cummins B, Auckland ML, Cummins P (1987) Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J 113:133–144.

    Article  Google Scholar 

  7. Katus HA, Remppis A, Looser S, Hallermeier K, Schefforld T, Kubler W (1989) Enzyme linked immunoassay of cardiac troponin T for the detection of acute myocardial infarction in patients. J Mol Cell Cardiol 21:1349–1353.

    Article  PubMed  CAS  Google Scholar 

  8. Nagai R, Ueda S, Yazaki Y (1979) Radioimmunoassay of cardiac myosin light chain II in the serum following experimental myocardial infarction. Biochem Biophys Res Commun 86:683–688.

    Article  PubMed  CAS  Google Scholar 

  9. Nagai R, Larson DM, Periasamy M (1988) Characterization of a mammalian smooth muscle myosin heavy chain cDNA clone and its expression in various smooth muscle types. Proc Natl Acad Sci USA 85:1047–105110.

    Article  PubMed  CAS  Google Scholar 

  10. Nagai R, Kuro-o M, Babij P, Periasamy M (1989) Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis. J Biol Chem 264:9734–9737.

    PubMed  CAS  Google Scholar 

  11. Kuro-o M, Nagai R, Tsuchimochi H et al. (1989) Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. J Biol Chem 264:18272–18275.

    PubMed  CAS  Google Scholar 

  12. Aikawa M, Silvam PN, Kuro-o M et al. (1993) Human smooth muscle myosin heavy chain isoforms as molecular markers for vascular development and atherosclerosis. Circ Res 73:1000–1012.

    PubMed  CAS  Google Scholar 

  13. Yanagisawa M, Hamada Y, Katsuragawa Y, Imamura M, Mikawa T, Masaki T (1987) Complete primary structure of vertebrate smooth muscle myosin heavy chain deduced from its complementary DNA sequence. J Mol Biol 198:143–157.

    Article  PubMed  CAS  Google Scholar 

  14. Miano JM, Cserjesi P, Ligon KL, Periasamy M, Olson EN (1994) Smooth muscle myosin heavy chain exclusively marks the smooth muscle lineage during mouse embryogenesis. Circ Res 75:803–812.

    PubMed  CAS  Google Scholar 

  15. Katoh H, Suzuki T, Hiroi Y et al. (1995) Diagnosis of aortic dissection by immunoassay for circulating smooth muscle myosin. Lancet 345:191–192.

    Article  PubMed  CAS  Google Scholar 

  16. Suzuki T, Katoh H, Watanabe M et al. (1996) A novel biochemical method for aortic dissection - the results of a prospective study using an immunoassay of smooth muscle myosin heavy chain. Circulation 93:1244–1249.

    PubMed  CAS  Google Scholar 

  17. Katoh H, Suzuki T, Yokomori K et al. (1995) A novel immunoassay of smooth muscle myosin heavy chain in serum. J Immunol Methods 185:57–63.

    Article  PubMed  CAS  Google Scholar 

  18. Suzuki T, Maekawa K, Morita H et al. Biochemical diagnosis of traumatic aortic rupture - using a serum assay of smooth muscle myosin heavy chain (submitted).

    Google Scholar 

  19. Sugi M, Kato H, Fujimoto M et al. (1987) Monoclonal antibodies to human beta interferon: characterization and application. Hybridoma 6:313–320.

    Article  PubMed  CAS  Google Scholar 

  20. Kohler G, Milstein C (1975) Continuous culture of fused cells secreting antibody of predefined specificity. Nature 256:495–497.

    Article  PubMed  CAS  Google Scholar 

  21. Miller DC (1985) Acute dissection of the aorta: continuing need for earlier diagnosis and treatment. Mod Con Cardiovasc Dis 54:51–55.

    Google Scholar 

  22. Anagnostopoulos CE, Prabhakar MJS, Kittle CF (1972) Aortic dissections and dissecting aneurysms. Am J Cardiol 30:263–273.

    Article  PubMed  CAS  Google Scholar 

  23. Wheat MW Jr (1980) Acute dissecting aneurysms of the aorta: diagnosis and treatment - 1979. Am Heart J 99:373–387.

    Article  PubMed  Google Scholar 

  24. Roberts WC (1981) Aortic dissection: anatomy, consequences, and causes. Am Heart J 101:195–214.

    Article  PubMed  CAS  Google Scholar 

  25. Hirst AE, Johns VJ Jr, Kime SW Jr (1958) Dissecting aneurysms of the aorta: a review of 505 cases. Medicine 37:217–279.

    Article  PubMed  Google Scholar 

  26. Cooke JP, Safford RE (1986) Progress in the diagnosis and management of aortic dissection. Mayo Clin Proc 61:147–153.

    PubMed  CAS  Google Scholar 

  27. Nienaber CA, von Kodolitsch Y, Nicolas V et al. (1993) The diagnosis of thoracic aortic dissection by noninvasive imaging procedures. N Engl J Med 328:1–9.

    Article  PubMed  CAS  Google Scholar 

  28. Spittell PC, Spittell JA Jr, Joyce JW et al. (1993) Clinical features and differential diagnosis of aortic dissection: experience with 236 cases (1980 through 1990). Mayo Clin Proc 68:642–651.

    PubMed  CAS  Google Scholar 

  29. Turney SZ, Rodriguez A (1990) Injuries to the great thoracic vessels. In: Turney SZ, Rodriguez A, Cowley RA (eds) Management of cardiothoracic trauma. Williams and Wilkins, Baltimore, pp 229–260.

    Google Scholar 

  30. Ben MY (1993) Rupture of the thoracic aorta by broadside impacts in road traffic and other collisions: further angiographic observations and preliminary autopsy findings. J Trauma 35:363–367.

    Article  Google Scholar 

  31. Smith RS, Chang FC (1986) Traumatic rupture of the aorta: still a lethal injury. Am J Surg 152:660–663.

    Article  PubMed  CAS  Google Scholar 

  32. Ochsner MJ, Hoffman AP, DiPasquale D et al. (1989) Pelvic fracture as an indicator of increased risk of thoracic aortic rupture. J Trauma 29:1376–1379.

    Article  PubMed  Google Scholar 

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Authors
  1. T. Suzuki
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Editors and Affiliations

  1. Klinik für Anästhesiologie und Intensivmedizin, Universität Graz, Auenbruggerplatz 29, A-8036, Graz, Austria

    W. F. List

  2. Kaiser-Franz-Joseph-Spital, Kundrastraße 3, A-1100, Wien, Austria

    M. M. Müller

  3. Dept. of Laboratory Medicine, McMaster University, 732 Barton Street, L8L 2X2, Hamilton, Canada

    M. J. McQueen

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© 1997 Springer-Verlag Berlin · Heidelberg

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Suzuki, T. (1997). Biochemical Diagnosis of Acute Aortic Damage - Diagnosis of Aortic Dissection and Traumatic Aortic Rupture Using an Immunoassay of Smooth Muscle Myosin Heavy Chain. In: List, W.F., Müller, M.M., McQueen, M.J. (eds) Advances in Critical Care Testing. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60735-6_1

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  • DOI: https://doi.org/10.1007/978-3-642-60735-6_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-62590-2

  • Online ISBN: 978-3-642-60735-6

  • eBook Packages: Springer Book Archive

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