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Definition
Microscale thermophoresis (MST) is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR laser-induced temperature change.
Introduction
Microscale thermophoresis (MST) (Dhont et al. 2007; Duhr and Braun 2006), as other methods used to characterize interaction parameters, utilizes differential detection of bound and unbound states of a given molecular specimen. Quantification of the absolute or relative amounts of bound and unbound state can be used to determine characteristic physical parameters that describe the chemical equilibrium of an interaction. Differentiation between molecular states (e.g., bound and unbound) of a molecule can be achieved by using techniques that measure size (gel electrophoresis, fluorescence correlation spectroscopy, fluorescence polarization), charge (electrophoretic mobility shift assay), or conformation...
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References
Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D (2010) Optical thermophoresis for quantifying the buffer dependence of aptamer binding. Angew Chem Int Ed Engl 49:2238–2241
Chapleau RR, Frey JS, Riddle DS, Ruiz ON, Mauzy CA (2015) Measuring single-domain antibody interactions with epitopes in jet fuel using microscale thermophoresis. Anal Lett 48(3):526–530
Dhont JKG, Wiegand S, Duhr S, Braun D (2007) Thermodiffusion of charged colloids: single-particle diffusion. Langmuir 23:1674–1683
Duhr S, Braun D (2006) Why molecules move along a temperature gradient. Proc Natl Acad Sci U S A 103:19678–19682
Koch S, de Wit JG, Vos I, Birkner JP, Gordiichuk P, Herrmann A, van Oijen AM, Driessen AJ (2016) Lipids activate SecA for high affinity binding to the SecYEG complex. J Biol Chem 291:22534–22543
Lou J, Finegan TΜ, Mohsen P, Hatton TA, Laibinis PE (1999) Fluorescence-based thermometry: principles and applications. Rev Anal Chem 18:235–284
Qu Y, Gharbi N, Yuan X, Olsen JR, Blicher P, Dalhus B, Brokstad KA, Lin B, Oyan AM, Zhang W et al (2016) Axitinib blocks Wnt/beta-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A 113:9339–9344
Rainard JM, Pandarakalam GC, McElroy SP (2018) Using microscale thermophoresis to characterize hits from high-throughput screening: a European lead factory perspective. SLAS Discov Adv Life Sci RD 23:225–241
Ross D, Gaitan M, Locascio LE (2001) Temperature measurement in microfluidic systems using a temperature-dependent fluorescent dye. Anal Chem 73:4117–4123
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Gupta, A.J., Duhr, S., Baaske, P. (2018). Microscale Thermophoresis (MST). In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_10063-1
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DOI: https://doi.org/10.1007/978-3-642-35943-9_10063-1
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Online ISBN: 978-3-642-35943-9
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