Regular Article
Synthesis and Characterization of Short-Chain Diacylphosphatidic Acids

https://doi.org/10.1006/jcis.1995.1059Get rights and content

Abstract

Several short-chain (dihexanoyl-, diheptanoyl-, and dioctanoyl-) phosphatidic acid (PA) molecules have been synthesized and characterized as water-soluble lipids for studies of cell activation and the interaction of negatively charged lipids with specific proteins. These species aggregate to form large polydisperse micelles. Critical micelle concentration (CMC) values of the dianions in 0.10 M NaCl are similar to those for the corresponding chain length phosphatidylcholine (PC) species. Surface properties suggest that the dianion has an area per molecule comparable to that of PC molecules, while the monoanion has a greatly reduced area, closer to twice the value of the two fatty acyl chains. This difference may be useful in understanding pH effects of protein-PA interactions.

References (0)

Cited by (12)

  • Role of tryptophan residues in interfacial binding of phosphatidylinositol-specific phospholipase C

    2002, Journal of Biological Chemistry
    Citation Excerpt :

    The decrease in fluorescence was the least for W242A and the double mutant. Upon micelle formation of diC6PA (the CMC depends on the ionic strength and pH of the medium and is likely to be 5–7 mm under these buffer conditions (22)), all proteins except W242A and the double mutant showed large increases in fluorescence consistent with micelle binding as well as active site binding of the PA molecule. This could suggest that Trp-242 not only senses micelle binding but contributes to the decrease in fluorescence as lipids bind to the active site.

View all citing articles on Scopus
View full text