Elsevier

Genomics

Volume 47, Issue 3, 1 February 1998, Pages 365-371
Genomics

Regular Article
Molecular Analysis of the cDNA and Genomic DNA Encoding Mouse RNA Helicase A

https://doi.org/10.1006/geno.1997.5139Get rights and content

Abstract

RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A. The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human andDrosophilahomologs, respectively. Using Southern blot analysis employing a mouse backcross panel, we have assigned the mouse RNA helicase A gene to chromosome 1, mapping near theD1Bir20locus at MGD position 67. Northern blot and primer extension analyses indicate that, although its level is variable, RNA helicase A appears to be expressed from a single transcription start site in all tissues tested. Sequence analysis of the upstream genomic DNA revealed that the promoter region lacks a TATA box and contains two high-affinity sites for Sp1, one ISRE, a binding site for interferon regulatory factor, and three AP2-binding sites. These findings suggest that the transcriptional regulation of the RNA helicase A gene is complex.

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    Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. U91922 and AF023530.

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