Plasmodium falciparum: Differential Display Analysis of Gene Expression during Gametocytogenesis

doi:10.1006/expr.2001.4669

Experimental Parasitology

Volume 99, Issue 4, December 2001, Pages 244-254

https://doi.org/10.1006/expr.2001.4669 Get rights and content

Abstract

Cui, L., Rzomp, K. A., Fan, Q., Martin, S. K., and Williams, J. 2001. Plasmodium falciparum: Differential display analysis of gene expression during gametocytogenesis. Experimental Parasitology99, 244–254. With the Plasmodium falciparum genome sequencing near completion, functional analysis of individual parasite genes has become the major task of the postgenomic era. Understanding the expression patterns of individual genes is the initial step toward this goal. In this report, we have examined gene expression during gametocytogenesis of the malaria parasite, P. falciparum, using a modified differential display (DD) method. The modifications of this method include adjusting the dNTP mix, using upstream primers with higher AT contents, and reducing the extension temperature of the polymerase chain reaction (PCR). With a combination of 16 arbitrary upstream primers and 3 one-base-anchored oligo(dT) primers, we have successfully cloned 80 unique cDNA tags from stage IV–V gametocytes. Further analysis by dot blots and semiquantitative reverse transcriptase-PCR showed that at least 49 cDNAs had induced or elevated levels of expression in gametocytes. These results indicate that this modified DD procedure is suitable for large-scale identification of developmentally regulated genes in the AT-rich Plasmodium genome.

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Regular ArticlePlasmodium falciparum: Differential Display Analysis of Gene Expression during Gametocytogenesis

Abstract

Molecular and Biochemical Parasitology

Parasitology Today

Molecular and Biochemical Parasitology

Microbes and Infection

Molecular and Biochemical Parasitology

Current Opinion in Cell Biology

Current Opinions in Immunology

Molecular and Biochemical Parasitology

Molecular and Biochemical Parasitology

Parasitology Today

Molecular and Biochemical Parasitology

Molecular and Biochemical Parasitology

Experimental Parasitology

Experimental Parasitology

Gene

Molecular and Biochemical Parasitology

Advances in Parasitology

Molecular and Biochemical Parasitology

Current Opinions in Microbiology

Molecular and Biochemical Parasitology

Sexual differentiation in malaria parasites

Annual Review of Microbiology

The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum

Nature

Gametocytes

Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum

Proceedings of the National Academy of Sciences USA

Molecular characterization of a prophenoloxidase cDNA from the malaria mosquito Anopheles stephensi

Insect Molecular Biology

Regular Article
Plasmodium falciparum: Differential Display Analysis of Gene Expression during Gametocytogenesis