Regular ArticleDensovirus of Aedes aegypti as an Expression Vector in Mosquito Cells
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Densonucleosis viruses (‘densoviruses’) for mosquito and pathogen control
2018, Current Opinion in Insect ScienceCitation Excerpt :Some experimentation with ncRNA expression has been conducted using a helper-transfection AaeDNV plasmid system to induce RNAi in A. albopictus C6/36 cells and larvae [52]. In this study, pUCA, a previously validated plasmid encoding the entire AaeDNV genome, was modified to create a recombinant plasmid lacking the VP gene and containing GFP fused to NS1 [48••,52]. An artificial intron containing a small hairpin RNA (shRNA) expression cassette sequence under the control of either an Anopheles.
Biology, genome organization, and evolution of parvoviruses in marine shrimp
2014, Advances in Virus ResearchMosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection
2008, VirologyCitation Excerpt :The first one to be described was the Aedes aegypti densonucleosis virus (AeDNV), which was isolated from a laboratory colony of A. aegypti mosquitoes (Buchatsky, 1989). AeDNV has been developed into a gene delivery and expression vector (Afanasiev et al., 1994, 1999; Allen-Miura et al., 1999). Several mosquito densonucleosis viruses have been sequenced (Afanasiev et al., 1991; Boublik et al., 1994; Chen et al., 2004; Paterson et al., 2005).