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Molecular Cloning and Expression Analysis of a Putative Nuclear Protein, SR-25,☆☆

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Abstract

We cloned a full-length mouse cDNA and its human homologue encoding a novel protein designated as “SR-25.” In Northern blot analysis, SR-25 mRNA was expressed in all organs tested, and relatively abundant in testis and thymus. Deduced amino acid sequences of mouse SR-25 and human SR-25 showed 77.7% identity. SR-25 has a serine-arginine repeat (SR repeat) and two types of amino acid clusters: a serine cluster and a highly basic cluster. Based on the presence of many nuclear localizing signals and a similarity to RNA splicing proteins, SR-25 is strongly suggested to be a nuclear protein and may contribute to RNA splicing.

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    Sequence data from this paper have been submitted to the DDBJ/EMBL/Genbank databases with the Accession Nos. AB035383 and AB035384.

    ☆☆

    Abbreviations used: FBS, fetal bovine serum; ORF, open reading frame; RACE, rapid amplification of cDNA ends; RT, reverse transcriptase; snRNP, small nuclear ribonucleoprotein particle; SR repeat, serine-arginine repeat; TdT, terminal deoxynucleotidyl transferase

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    To whom correspondence should be addressed at Division of Genetic Information, Institute for Genome Research, University of Tokushima, Tokushima, 770-8503, Japan. Fax: +81-88-631-9476. E-mail: [email protected].

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