Notes & TipsPhosphohistidine Analysis Using Reversed-Phase Thin-Layer Chromatography☆
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Chapter 14 Protein Histidine Phosphorylation
2008, Comprehensive Analytical ChemistryCitation Excerpt :There is thin-layer electrophoresis, usually on cellulose plates [23,38] although historically electrophoretic separation has also been done on paper [110]. Recently, reverse-phase thin-layer chromatography has been employed for the analysis of phosphohistidine-containing phosphoprotein hydrolysates [40,109,111]. Figure 6 shows a reverse-phase thin-layer chromatogram of histone H4 phosphorylated by a partially purified preparation of yeast HHK.
Mammalian histidine kinases
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This work was supported by a grant from the National Science Foundation to C. W. Turck (MCB 9727920).
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