Regular ArticleAxon Regeneration in Vitro on Physiologically Relevant Substrata
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cGMP promotes neurite outgrowth and growth cone turning and improves axon regeneration on spinal cord tissue in combination with cAMP
2009, Brain ResearchCitation Excerpt :We therefore presented embryonic DRG neurons with a gradient of 8-Br-cGMP and found that they also showed a significant attraction to the gradient with a mean turning angle of + 14.8 ± 4.5° (N = 12 growth cones; Figs. 2G, H and I). The cryoculture in vitro model of axon regeneration provides a convenient mechanism for assaying neurite outgrowth in an environment that maintains many of the growth inhibiting properties of the mature nervous system in vivo (Shewan et al., 1994, 1995; Murray and Shewan, 2008). Dissociated neonatal and adult DRG neurons seeded on sections of adult rat spinal cord grow very poorly but embryonic neurons are able to grow extensive neurites (Shewan et al., 1995).
Cryosections of pre-irradiated adult rat spinal cord tissue support axonal regeneration in vitro
2000, International Journal of Developmental NeuroscienceCitation Excerpt :Taken together, these observations suggest that the microenvironment following X-irradiation may not be as inhibitory to axonal regeneration as that provided by the normal spinal cord. To examine the mechanisms of axonal regeneration, we have previously used a cryoculture technique that allows many of the complexities of events occurring in vivo to be circumvented [5,39,40]. In this technique, dissociated neurons are plated onto cryosections of various tissues to determine their capacity for neurite extension in vitro.
Maturation of the mammalian dorsal root entry zone - From entry to no entry
1997, Trends in NeurosciencesAn in vitro model of the rat dorsal root entry zone reveals developmental changes in the extent of sensory axon growth into the spinal cord
1996, Molecular and Cellular Neurosciences