Regular ArticleComparison of Fluorescence, 31P NMR, and 7Li NMR Spectroscopic Methods for Investigating Li+/Mg2+ Competition for Biomolecules
References (18)
- et al.
J. Inorg. Biochem.
(1991) - et al.
FEBS Lett.
(1989) - et al.
Biochem. Biophys. Res. Commun.
(1986) - et al.
- et al.
Lithium
(1992) - et al.
Lithium
(1994) - et al.
Biochemistry
(1994) Miner. Electrolyte Metab.
(1993)
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How does the mood stabilizer lithium bind ATP, the energy currency of the cell: Insights from solid-state NMR
2020, Biochimica et Biophysica Acta - General SubjectsTreatment of trauma with lithium to forestall the development of posttraumatic stress disorder by pharmacological induction of a mild transient amnesia
2013, Medical HypothesesCitation Excerpt :The lithium ion, the active principle in lithium carbonate, has a crystal ionic radius of .60 Å, similar to that of the magnesium ion at .65 Å [32]. Like magnesium, the lithium ion is positively charged, and because both molecules have similar properties, lithium can bind to and inhibit magnesium dependent molecules, including receptors and enzymes [32–35]. The diverse number of receptors and enzymes affected by magnesium throughout the body makes the identification of the therapeutic target(s) of lithium more challenging in comparison to other psychotropic medications that have more clearly defined sites of action.
Multinuclear NMR studies of the interaction of metal ions with adenine-nucleotides
2008, Coordination Chemistry ReviewsEffects of Li<sup>+</sup> transport and intracellular binding on Li <sup>+</sup>/Mg<sup>2+</sup> competition in bovine chromaffin cells
2004, Biochimica et Biophysica Acta - Molecular Cell ResearchCitation Excerpt :The [Mg2+]f increases when Li+ enters the cells observed by fluorescence spectroscopy confirms the capacity of Li+ to displace Mg2+ from its intracellular binding sites. However, it is confirmed that they are too small to be detected by the much less sensitive 31P NMR spectroscopic methods [22–26]. The [Mg2+]f increases estimated in the present work are much smaller than those obtained for these cells in suspension [25].
Competition between lithium and magnesium ions for the G-protein transducin in the guanosine 5<sup>′</sup>-diphosphate bound conformation
2004, Journal of Inorganic BiochemistryCitation Excerpt :In this study, we used 7Li T1 relaxation measurements and fluorescence spectroscopy with the Mg2+ fluorophore, furaptra, to examine the Li+/Mg2+ competition hypothesis in three model systems: the purified G-protein Gt, stripped ROS membranes with bound Gt (ROS-T), and stripped ROS membranes without bound Gt (SROS). Since the observed 7Li T1 values are a weighted average of the free and bound 7Li T1 values, they represent a function of free Li+ concentrations and, indirectly, a function of free Mg2+ concentrations [27,35,43], because Li+ and Mg2+ compete for the same Mg2+ binding sites. Therefore, this method is sensitive to both Li+-induced Mg2+ displacement and Mg2+-induced Li+ displacement.
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