Elsevier

Analytical Biochemistry

Volume 235, Issue 2, 15 March 1996, Pages 195-201
Analytical Biochemistry

Regular Article
Tetracycline-Controlled Gene Expression System Achieves High-Level and Quantitative Control of Gene Expression

https://doi.org/10.1006/abio.1996.0112Get rights and content
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Abstract

The tetracycline-controlled gene expression system utilizes the control elements of the tetracycline resistance operon encoded in Tn10 ofEscherichia colito control gene expression in eukaryotic cells. Here we demonstrate the quantitative control of the expression of the luciferase gene, dihydrofolate reductase gene, andbcl-2gene in HeLa S3 or Chinese hamster ovary AA8 cells using the tetracycline-controlled gene expression system. Regardless of the host cell lines or the genes being expressed, there is a common range of tetracycline concentration within which the expression of genes is most sensitively regulated. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system is higher than that of the wild-type CMV promoter/enhancer-driven system. Nonetheless, careful selection of stably transfected clones is necessary to achieve the optimally regulated gene expression using this system.

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