Regular ArticleGene Expression Profile of Human Bone Marrow Stromal Cells: High-Throughput Expressed Sequence Tag Sequencing Analysis
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2016, CytotherapyCitation Excerpt :The characterization of BMSCs remains especially difficult because of the lack of a definitive and unique cellular marker. A number of studies including clonal assays followed by transcriptomic analyses have been published, highlighting the difficulty of properly characterizing the cellular composition of MSC [9,11–14]. The International Society for Cellular Therapy therefore proposed three minimal criteria for the definition of cultured MSCs: (i) plastic adherence; (ii) expression of CD73, CD90 and CD105, the lack of CD34, CD11b or CD14, CD19 or CD79α, CD45 and HLA-DR expression; and (c) their trilineage differentiation potential into adipocytes, chondrocytes and osteoblasts [15].
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2013, Biotechnology AdvancesCitation Excerpt :In addition, because their phenotype may be affected by the culture medium, the plating density and the oxygen tension, the precise phenotype of cultured MSC is still debated, and their identification remains ambiguous (Gnecchi et al., 2012). Numerous studies including clonal assays followed by transcriptomic analyses have been carried out for molecular characterization of MSC and demonstrated the difficulty of molecular characterization of MSC (Jia et al., 2002; Kuznetsov et al., 1997; Muraglia et al., 2000; Panepucci et al., 2004; Russell et al., 2011; Silva et al., 2003; Tremain et al., 2001; Wagner et al., 2005, 2006; Wislet-Gendebien et al., 2012a). The limitations of clonal selection are that not all cells can be clonally expanded and cellular properties could be altered during clonal expansion (Wislet-Gendebien et al., 2012b).
Panax notoginseng saponins promotes proliferation and osteogenic differentiation of rat bone marrow stromal cells
2011, Journal of EthnopharmacologyCitation Excerpt :Thus, restoring the balance between osteoblasts and adipocytes is a key goal of pharmacological intervention in osteoporosis (Fu et al., 2008). It has been proposed that undifferentiated BMSCs simultaneously coexpress genes characteristic of a number of mesenchymal lineages, including osteoblasts and adipocytes (Jia et al., 2002), the differentiation commitment of BMSCs is controlled by a series of transcription regulators. Cbfa1 and PPARγ2 are two key transcription factors that drive BMSCs to undergo osteoblast and adipocyte differentiation, respectively (Zhang et al., 2008).
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2007, CytotherapyCitation Excerpt :Their relative over-expression in MSC may be a consequence of the rapid morphologic changes required for differentiation. The gene expression profile of BM-derived MSC has been investigated using serial analysis of gene expression (SAGE) [43] and restriction fragment differential display (RFDD) [44]. Jeong et al. [45] performed a comparative analysis of gene expression between UCB-derived MNC and MSC using microarray techniques with RT-PCR confirmation.
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