Elsevier

Genomics

Volume 71, Issue 1, 1 January 2001, Pages 89-100
Genomics

Regular Article
Structure of the Human Zinc Finger Protein HIVEP3: Molecular Cloning, Expression, Exon–Intron Structure, and Comparison with Paralogous Genes HIVEP1 and HIVEP2

https://doi.org/10.1006/geno.2000.6425Get rights and content

Abstract

Here we report the cloning and characterization of HIVEP3, the newest member in the human immunodeficiency virus type 1 enhancer-binding protein family that encodes large zinc finger proteins and regulates transcription via the κB enhancer motif. The largest open reading frame of HIVEP3 contains 2406 aa. and is ∼80% identical to the mouse counterpart. The HIVEP3 gene is located in the chromosomal region 1p34 and is at least 300 kb with 10 exons. RNA studies show that multiple HIVEP3 transcripts are differentially expressed and regulated. Additionally, transcription termination occurs in the ultimate exon, exon 10, or in exon 6. Therefore, HIVEP3 may produce protein isoforms that contain or exclude the carboxyl DNA binding domain and the leucine zipper by alternative RNA splicing and differential polyadenylation. Sequence homologous to HIVEP3 exon 6 is not found in mouse nor are the paralogous genes HIVEP1 and HIVEP2. Zoo-blot analysis suggests that sequences homologous to the human exon 6 are present only in primates and cow. Therefore, a foreign DNA harboring a termination exon likely was inserted into the HIVEP3 locus relatively recently in evolution, resulting in the acquisition of novel gene regulatory mechanisms as well as the generation of structural and functional diversity.

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    Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. AF278765 and AF278766.

    1

    To whom correspondence should be addressed at Room S2077, Davis Medical Center, Department of Internal Medicine, The Ohio State University, 480 West Ninth Avenue, Columbus, OH 43210. Telephone: (614) 293-3042. Fax: (614) 293-5631. E-mail: [email protected].

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