An Integrated Physical and Genetic Map Spanning Chromosome Band 10q24

doi:10.1006/geno.1997.4809

Genomics

Volume 43, Issue 1, 1 July 1997, Pages 85-88

https://doi.org/10.1006/geno.1997.4809 Get rights and content

Abstract

Chromosome band 10q24 is rich in genes involved in development, tumorigenesis, neurological disorders, hormone metabolism, and environmentally induced disease susceptibility. We have constructed an STS-based integrated physical and genetic map of 10q24 derived from the CEPH–Généthon mega-YAC contig data for this region. This map consists of 42 fluorescencein situhybridization-mapped overlapping CEPH mega-YACs spanning approximately 15 Mb to which 49 STS markers have been assigned, including 24 Généthon CA repeat genetic markers, 10 known gene loci from the 10q24 region (IFI56, IDE, PDE6C, RBP4, CYP2C, CD39, DNTT, GOT1, WNT8B,andPAX2) and 11 additional expressed sequences of unknown function.

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It overlaps the larger genomic region, spanning 12 cM at 10q23.3–q24.2, recently linked to a form of autosomal dominant spastic paraplegia (SPG9; Seri et al., 1999). To identify candidate genes for these diseases, we constructed a YAC map of the 10q24 region (Gray et al., 1997; Siebert et al., unpublished) and placed on it numerous brain expressed sequence tags (ESTs; Nobile et al., 1998; Lo Nigro et al., 2000). Subsequent expression analysis of these ESTs led to the identification of several putative genes selectively or predominantly expressed in the brain (Nobile and Pitzalis, 1999), which may be involved in neurological disorders.
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¹: Present address: SmithKline Beecham Pharmaceuticals, Biopharmaceutical R&D, New Frontiers Science Park (North), Harlow, Essex CM10 5AW, UK.

²: To whom correspondence should be addressed. Telephone: 01279 627225. Fax: 01279 622500. E-mail: [email protected].

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SHORT COMMUNICATIONAn Integrated Physical and Genetic Map Spanning Chromosome Band 10q24

Abstract

Genomics

Genomics

Genomics

IMAGE: Molecular integration of the analysis of the human genome and its expression

C. R. Acad. Sci. III

A yac contig map of the human genome

Nature

A first generation physical map of the human genome

Nature

A comprehensive genetic-map of the human genome based on 5,264 microsatellites

Nature

Loss of the chromosomal region 10q23–25 in prostate cancer

Cancer Res.

Deregulation of a homeobox gene, HOX11, by the t(10;14) in T cell leukemia

Science

An STS-based map of the human genome

Science

A new bacteriophage P1-derived vector for the propagation of large human DNA fragments

Nature Genet.

Allelic loss on chromosome 10 in prostate adenocarcinoma

Cancer Res.

SHORT COMMUNICATION
An Integrated Physical and Genetic Map Spanning Chromosome Band 10q24