Regulation of the Stem Cell–Host Immune System Interplay Using Hydrogel Coencapsulation System with an Anti-Inflammatory Drug
Corresponding Author
Alireza Moshaverinia
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
E-mail: [email protected], [email protected]Search for more papers by this authorChider Chen
School of Dental Medicine, University of Pennsylvania, 240 South 40th Street, Philadelphia, PA, 19104 USA
Search for more papers by this authorXingtian Xu
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorSahar Ansari
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorHomayoun H. Zadeh
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorScott R. Schricker
College of Dentistry, Ohio State University, 305 W 12th Ave, Columbus, OH, 43210 USA
Search for more papers by this authorMichael L. Paine
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorJanet Moradian-Oldak
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorAli Khademhosseini
Biomaterials Innovation Research Center, Harvard Medical School, 65 Landsdowne St, Rm 252, Cambridge, MA, 02139 USA
Search for more papers by this authorMalcolm L. Snead
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorCorresponding Author
Songtao Shi
School of Dental Medicine, University of Pennsylvania, 240 South 40th Street, Philadelphia, PA, 19104 USA
E-mail: [email protected], [email protected]Search for more papers by this authorCorresponding Author
Alireza Moshaverinia
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
E-mail: [email protected], [email protected]Search for more papers by this authorChider Chen
School of Dental Medicine, University of Pennsylvania, 240 South 40th Street, Philadelphia, PA, 19104 USA
Search for more papers by this authorXingtian Xu
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorSahar Ansari
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorHomayoun H. Zadeh
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorScott R. Schricker
College of Dentistry, Ohio State University, 305 W 12th Ave, Columbus, OH, 43210 USA
Search for more papers by this authorMichael L. Paine
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorJanet Moradian-Oldak
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorAli Khademhosseini
Biomaterials Innovation Research Center, Harvard Medical School, 65 Landsdowne St, Rm 252, Cambridge, MA, 02139 USA
Search for more papers by this authorMalcolm L. Snead
Center for Craniofacial Molecular Biology (CCMB), Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St, Los Angeles, CA, 90033 USA
Search for more papers by this authorCorresponding Author
Songtao Shi
School of Dental Medicine, University of Pennsylvania, 240 South 40th Street, Philadelphia, PA, 19104 USA
E-mail: [email protected], [email protected]Search for more papers by this authorAbstract
The host immune system is known to influence mesenchymal stem cell (MSC)-mediated bone tissue regeneration. However, the therapeutic capacity of hydrogel biomaterial to modulate the interplay between MSCs and T-lymphocytes is unknown. Here it is shown that encapsulating hydrogel affects this interplay when used to encapsulate MSCs for implantation by hindering the penetration of pro-inflammatory cells and/or cytokines, leading to improved viability of the encapsulated MSCs. This combats the effects of the host pro-inflammatory T-lymphocyte-induced nuclear factor kappaB pathway, which can reduce MSC viability through the CASPASE-3 and CASPASE-8 associated proapoptotic cascade, resulting in the apoptosis of MSCs. To corroborate rescue of engrafted MSCs from the insult of the host immune system, the incorporation of the anti-inflammatory drug indomethacin into the encapsulating alginate hydrogel further regulates the local microenvironment and prevents pro-inflammatory cytokine-induced apoptosis. These findings suggest that the encapsulating hydrogel can regulate the MSC-host immune cell interplay and direct the fate of the implanted MSCs, leading to enhanced tissue regeneration.
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