Abstract
Studies in mouse and human have established that the exon encoding the neuroendocrine secretory protein Nesp55 is transcribed exclusively from the maternal allele. To study the imprinting status of Nesp55 in cattle, we examined transcripts of this exon in a wide range of tissues from four fetuses and the dam of one of the fetuses (not all tissues were examined in all subjects). A polymorphic site was used to determine transcript status as monoallelic or biallelic. The expression analysis was by direct sequencing of RT-PCR products amplified from the tissues. Nesp55 transcripts were detected in many tissues, in contrast to previous studies in which Nesp55 was detected by immunoassays in endocrine and nervous tissues only. In all five individuals, Nesp55 showed monoallelic expression. In one of the fetuses, parental origin of the expressed allele could not be determined; in the others monoallelic expression was clearly maternal. Thus, bovine Nesp55 is maternally expressed like its counterparts in mouse and human.
Similar content being viewed by others
References
R Bunzel I Blümcke S Cichon S Normann J Schramm et al. (1998) ArticleTitlePolymorphic imprinting of the serotonin-2A (5-HT2A) receptor gene in human adult brain Brain Res Mol Brain Res 59 90–92 Occurrence Handle10.1016/S0169-328X(98)00146-6 Occurrence Handle1:CAS:528:DyaK1cXkvFOksbc%3D Occurrence Handle9729300
C Charlier K Segers D Wagenaar L Karim S Berghmans et al. (2001) ArticleTitleHuman–ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8 Genome Res 11 850–862 Occurrence Handle10.1101/gr.172701 Occurrence Handle1:CAS:528:DC%2BD3MXjs1Wmu78%3D Occurrence Handle11337479
S Croteau MC Charron KE Latham AK Naumova (2003) ArticleTitleAlternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos Mamm Genome 14 231–241 Occurrence Handle10.1007/s00335-002-2244-x Occurrence Handle1:CAS:528:DC%2BD3sXisleqt78%3D Occurrence Handle12682775
DJ Koning Particlede AP Rattink B Harlizius JA Arendonk Particlevan EW Brascamp et al. (2000) ArticleTitleGenome-wide scan for body composition in pigs reveals important role of imprinting Proc Natl Acad Sci USA 97 7947–7950 Occurrence Handle10.1073/pnas.140216397 Occurrence Handle10859367
BE Hayward V Moran L Strain DT Bonthron (1998) ArticleTitleBidirectional imprinting of a single gene: GNAS1 encodes maternally, paternally, and biallelically derived proteins Proc Natl Acad Sci USA 95 15475–15480 Occurrence Handle10.1073/pnas.95.26.15475 Occurrence Handle1:CAS:528:DyaK1MXhvFarsQ%3D%3D Occurrence Handle9860993
BE Hayward A Barlier M Korbonits AB Grossman P Jacquet et al. (2001) ArticleTitleImprinting of the G(s)alpha gene GNAS1 in the pathogenesis of acromegaly J Clin Invest 107 R31–36 Occurrence Handle1:CAS:528:DC%2BD3MXitVGnsL0%3D Occurrence Handle11254676
R Holmes C Williamson J Peters P Denny C Wells (2003) ArticleTitleA comprehensive transcript map of the mouse Gnas imprinted complex Genome Res 13 1410–1415 Occurrence Handle10.1101/gr.955503 Occurrence Handle1:CAS:528:DC%2BD3sXltlSkt7c%3D Occurrence Handle12819140
R Ischia P Lovisetti–Scamihorn R Hogue–Angeletti M Wolkersdorfer H Winkler et al. (1997) ArticleTitleMolecular cloning and characterization of NESP55, a novel chromogranin-like precursor of a peptide with 5-HT1B receptor antagonist activity J Biol Chem 272 11657–11662 Occurrence Handle10.1074/jbc.272.17.11657 Occurrence Handle1:CAS:528:DyaK2sXjtValsLw%3D Occurrence Handle9111083
JK Killian CM Nolan AA Wylie T Li TH Vu et al. (2001) ArticleTitleDivergent evolution in M6P/IGF2R imprinting from the Jurassic to the Quaternary Hum Mol Genet 10 1721–1728 Occurrence Handle10.1093/hmg/10.17.1721 Occurrence Handle1:CAS:528:DC%2BD3MXntFCitL0%3D Occurrence Handle11532981
T Li TH Vu ZL Zeng BT Nguyen BE Hayward et al. (2000) ArticleTitleTissue-specific expression of antisense and sense transcripts at the imprinted Gnas locus Genomics 69 295–304 Occurrence Handle10.1006/geno.2000.6337 Occurrence Handle1:CAS:528:DC%2BD3cXosVCru7Y%3D Occurrence Handle11056047
P Lovisetti–Scamihorn R Fischer–Colbrie B Leitner G Scherzer H Winkler (1999) ArticleTitleRelative amounts and molecular forms of NESP55 in various bovine tissues Brain Res 829 99–106 Occurrence Handle10.1016/S0006-8993(99)01345-1 Occurrence Handle1:CAS:528:DyaK1MXivFehs70%3D Occurrence Handle10350534
Y Muramatsu HY Lejukole Y Tanigu chi BA Konfortov T Yamada et al. (2002) ArticleTitleChromosomal assignments of expressed sequence tags for ACTG1, AHSG, COL1A1, GNAS1, and RPLP1 expressed abundantly in the bovine foetus Anim Genet 33 230–231 Occurrence Handle10.1046/j.1365-2052.2002.t01-1-00876.x Occurrence Handle1:CAS:528:DC%2BD38Xlt1KjsLg%3D Occurrence Handle12030931
SK Murphy RL Jirtle (2003) ArticleTitleImprinting evolution and the price of silence BioEssays 25 577–588 Occurrence Handle10.1002/bies.10277 Occurrence Handle1:CAS:528:DC%2BD3sXltlGkurY%3D Occurrence Handle12766947
C Nezer L Moreau B Brouwers W Coppieters J Detilleux et al. (1999) ArticleTitleAn imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs Nat Genet 21 155–156 Occurrence Handle10.1038/5935 Occurrence Handle1:CAS:528:DyaK1MXpsVCltg%3D%3D Occurrence Handle9988262
J Peters SF Wroe CA Wells HJ Miller D Bodle et al. (1999) ArticleTitleA cluster of oppositely imprinted transcripts at the Gnas locus in the distal imprinting region of mouse chromosome 2 Proc Natl Acad Sci USA 96 3830–3835 Occurrence Handle10.1073/pnas.96.7.3830 Occurrence Handle1:CAS:528:DyaK1MXjslChs7w%3D Occurrence Handle10097123
W Reik F Santos W Dean (2003) ArticleTitleMammalian epigenomics: reprogramming the genome for development and therapy Theriogenology 59 21–32 Occurrence Handle10.1016/S0093-691X(02)01269-4 Occurrence Handle1:CAS:528:DC%2BD38XpslSmurs%3D Occurrence Handle12499015
SJ Rickard LC Wilson (2003) ArticleTitleAnalysis of GNAS1 and overlapping transcripts identifies the parental origin of mutations in patients with sporadic Albright hereditary osteodystrophy and reveals a model system in which to observe the effects of splicing mutations on translated and untranslated messenger RNA Am J Hum Genet 72 961–974 Occurrence Handle10.1086/374566 Occurrence Handle12624854
JD Robishaw DW Russell BA Harris MD Smigel AG Gilman (1986) ArticleTitleDeduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of denylate cyclase Proc Natl Acad Sci USA 83 1251–1255 Occurrence Handle1:CAS:528:DyaL28XitVWgu78%3D Occurrence Handle3081893
J Schlapfer DS Gallagher SuffixJr JD Burzlaff JE Womack DM Stelly et al. (1998) ArticleTitleComparative mapping of bovine chromosome 13 by fluorescence in situ hybridization Anim Genet 29 265–272 Occurrence Handle10.1046/j.1365-2052.1998.00321.x Occurrence Handle1:CAS:528:DyaK1cXlvVCnt70%3D Occurrence Handle9745664
RJ Smith W Dean G Konfortova G Kelsey (2003) ArticleTitleIdentification of novel imprinted genes in a genome-wide screen for maternal methylation Genome Res 13 558–569 Occurrence Handle10.1101/gr.781503 Occurrence Handle1:CAS:528:DC%2BD3sXjt1SqtrY%3D Occurrence Handle12670997
Acknowledgments
The author thanks Professor Morris Soller for helpful discussions and critical reading of the manuscript. The author also thanks Valerie Schutzkus for very helpful technical assistance. This research was supported by a startup package grant from the University of Wisconsin.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Khatib, H. Imprinting of Nesp55 gene in cattle. Mamm Genome 15, 663–667 (2004). https://doi.org/10.1007/s00335-004-2331-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00335-004-2331-2